Western Blot (WB)
(GPHB5 Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the GPHB5 antibody detected the GPHB5 protein (arrow).)
Rabbit anti-Human GPHB5 Polyclonal Antibody | anti-GPHB5 antibody
GPHB5, CT (GPHB5, GPB5, ZLUT1, Glycoprotein hormone beta-5, Thyrostimulin subunit beta) (PE)
Gene Names
GPHB5; B5; GPB5; ZLUT1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GPHB5; Polyclonal Antibody; CT (GPHB5; GPB5; ZLUT1; Glycoprotein hormone beta-5; Thyrostimulin subunit beta) (PE); Thyrostimulin subunit beta; anti-GPHB5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-GPHB5 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
GPHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the C-terminal region of human GPHB5.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(GPHB5 Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the GPHB5 antibody detected the GPHB5 protein (arrow).)
Western Blot (WB)
(GPHB5 Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the GPHB5 antibody detected the GPHB5 protein (arrow).)
Immunohistochemistry (IHC)
(GPHB5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPHB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GPHB5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPHB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-GPHB5 antibody
GPHB5 is a cystine knot-forming polypeptide and a subunit of the dimeric glycoprotein hormone family (Hsu et al., 2002 [PubMed 12089349]).
Product Categories/Family for anti-GPHB5 antibody
References
Suzuki, C., et al. Endocrinology 150(5):2237-2243(2009). Okajima, Y., et al. Regul. Pept. 148 (1-3), 62-67 (2008): Okada, S.L., et al. Mol. Endocrinol. 20(2):414-425(2006). Sudo, S., et al. Endocrinology 146(8):3596-3604(2005). Heilig, R., et al. Nature 421(6923):601-607(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,232 Da
NCBI Official Full Name
glycoprotein hormone beta-5
NCBI Official Synonym Full Names
glycoprotein hormone beta 5
NCBI Official Symbol
GPHB5
NCBI Official Synonym Symbols
B5; GPB5; ZLUT1
NCBI Protein Information
glycoprotein hormone beta-5; glycoprotein beta 5; thyrostimulin subunit beta; glycoprotein hormone beta subunit
UniProt Protein Name
Glycoprotein hormone beta-5
Protein Family
UniProt Gene Name
GPHB5
UniProt Synonym Gene Names
GPB5; ZLUT1
UniProt Entry Name
GPHB5_HUMAN
NCBI Description
GPHB5 is a cystine knot-forming polypeptide and a subunit of the dimeric glycoprotein hormone family (Hsu et al., 2002 [PubMed 12089349]).[supplied by OMIM]
Uniprot Description
GPHB5: Stimulates the thyroid. Binds and activates THSR, leading to increased cAMP production. Belongs to the glycoprotein hormones subunit beta family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q23.2
Cellular Component: extracellular region
Molecular Function: hormone activity
Research Articles on GPHB5
Similar Products
Product Notes
The GPHB5 gphb5 (Catalog #AAA6303534) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPHB5, CT (GPHB5, GPB5, ZLUT1, Glycoprotein hormone beta-5, Thyrostimulin subunit beta) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPHB5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GPHB5 gphb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPHB5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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