Rabbit GPER1 / GPR30 Polyclonal Antibody | anti-GPER1 antibody

Rabbit Polyclonal to Human GPER1 / GPR30

Cat. Num. AAA244990

• Gene Names: GPER1; mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br
• Reactivity: Dog, Gorilla, Human, Monkey; Predicted Reactivity: Rat, Horse (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: GPER1 / GPR30; Polyclonal Antibody; Rabbit Polyclonal to Human GPER1 / GPR30; Anti-GPER1 / GPR30 Antibody (C-Terminus) IHC-plus; GPER1; CMKRL2; CEPR; FEG-1; G-protein coupled receptor 30; G protein-coupled receptor 30; GPCR-Br; GPER; Heptahelix receptor; LERGU; LyGPR; MER; GPR30; IL8-related receptor DRY12; LERGU2; Chemokine receptor-like 2; DRY12; Membrane estrogen receptor; Human GPER1; anti-GPER1 antibody

• Host: Rabbit
• Reactivity: Dog, Gorilla, Human, Monkey; Predicted Reactivity: Rat, Horse (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 375

• Applicable Applications for anti-GPER1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF, IHC-P (10 - 21 ug/ml), WB
• Target Species: Human
• Immunogen Description: Synthetic 18 amino acid peptide from C-terminus of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog (100%); Rat, Horse (94%); Mouse, Hamster (89%); Elephant, Bovine (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: GPER1 / GPR30 antibody was raised against synthetic 18 amino acid peptide from C-terminus of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog (100%); Rat, Horse (94%); Mouse, Hamster (89%); Elephant, Bovine (83%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-GPER1 / GPR30 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-GPR30 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-GPER1 / GPR30 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-GPER1 antibody

GPR30 is a transmembrane intracellular estrogen receptor (Revankar et al. 2005). Progestin inhibits growth in breast cancer cells through GPR30.

Product Categories/Family for anti-GPER1 antibody

Family: GPCR; Subfamily: Transmembrane Estrogen Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 4504091
• NCBI GeneID: 2852
• NCBI Accession #: NP_001496.1
• NCBI GenBank Nucleotide #: NM_001505.2
• UniProt Accession #: Q99527; O00143; O43494; Q13631; Q6FHL1; Q96F42; Q99981; A8K6C5; B5BUJ1
• Molecular Weight: 42,248 Da
• NCBI Official Full Name: G-protein coupled estrogen receptor 1
• NCBI Official Synonym Full Names: G protein-coupled estrogen receptor 1
• NCBI Official Symbol: GPER1
• NCBI Official Synonym Symbols: mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br
• NCBI Protein Information: G-protein coupled estrogen receptor 1; heptahelix receptor; chemokine receptor-like 2; IL8-related receptor DRY12; membrane estrogen receptor; G protein-coupled receptor 30; chemoattractant receptor-like 2; leucine rich protein in GPR30 3'UTR; lymphocyte-derived G-protein coupled receptor; constitutively expressed peptide-like receptor; flow-induced endothelial G-protein coupled receptor 1
• UniProt Protein Name: G-protein coupled estrogen receptor 1
• Protein Family: G-protein coupled estrogen receptor
• UniProt Gene Name: GPER1
• UniProt Synonym Gene Names: CEPR; CMKRL2; DRY12; GPER; GPR30; FEG-1; LYGPR; mER
• UniProt Entry Name: GPER1_HUMAN

NCBI Description

This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

GPER1: Receptor for estrogen. Belongs to the G-protein coupled receptor 1 family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: Golgi apparatus; integral to plasma membrane; early endosome; dendrite; nuclear envelope; trans-Golgi network; recycling endosome; perinuclear region of cytoplasm; cytoplasm; keratin filament; intracellular; dendritic shaft; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; endoplasmic reticulum; postsynaptic density; Golgi membrane; presynaptic membrane; axon; mitochondrial membrane; plasma membrane; presynaptic active zone; nerve terminal; cell junction; nucleus

Molecular Function: G-protein coupled receptor activity; protein binding; estrogen receptor activity; mineralocorticoid receptor activity; chromatin binding; steroid binding

Biological Process: positive regulation of apoptosis; generation of action potential; positive regulation of caspase activity; nuclear fragmentation during apoptosis; negative regulation of DNA metabolic process; positive regulation of vasodilation; positive regulation of neurotransmitter secretion; negative regulation of leukocyte activation; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; inflammatory response; steroid hormone receptor signaling pathway; positive regulation of neurogenesis; cytosolic calcium ion homeostasis; negative regulation of lipid biosynthetic process; positive regulation of insulin secretion; positive regulation of inositol trisphosphate biosynthetic process; cell cycle; apoptotic chromosome condensation; negative regulation of fat cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; mineralocorticoid receptor signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; innate immune response; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of protein amino acid phosphorylation; positive regulation of cell migration