Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GPD2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Rabbit GPD2 Polyclonal Antibody | anti-GPD2 antibody
GPD2 Rabbit pAb
Gene Names
GPD2; GDH2; GPDM; mGPDH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
GPD2; Polyclonal Antibody; GPD2 Rabbit pAb; GDH2; GPDM; mGPDH; anti-GPD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
SQFAHYRRKQMNLAYVKAADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIMLPVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVRVSGARCKDVLTGQEFDVRAKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSP
Applicable Applications for anti-GPD2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human GPD2 (NP_000399.3).
Cellular Location
Mitochondrion
Positive Samples
THP-1, Mouse skeletal muscle
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GPD2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GPD2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Related Product Information for anti-GPD2 antibody
Background: The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.
Product Categories/Family for anti-GPD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
727
NCBI Official Full Name
glycerol-3-phosphate dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
NCBI Official Symbol
GPD2
NCBI Official Synonym Symbols
GDH2; GPDM; mGPDH
NCBI Protein Information
glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; mtGPD; GPDH-M; mitochondrial glycerophosphate dehydrogenase
UniProt Protein Name
Glycerol-3-phosphate dehydrogenase, mitochondrial
Protein Family
UniProt Gene Name
GPD2
UniProt Synonym Gene Names
GPD-M; GPDH-M
UniProt Entry Name
GPDM_HUMAN
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Product Notes
The GPD2 gpd2 (Catalog #AAA9142296) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPD2 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GPD2 gpd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SQFAHYRRKQ MNLAYVKAAD CISEPVNREP PSREAQLLTL QNTSEFDILV IGGGATGSGC ALDAVTRGLK TALVERDDFS SGTSSRSTKL IHGGVRYLQK AIMKLDIEQY RMVKEALHER ANLLEIAPHL SAPLPIMLPV YKWWQLPYYW VGIKLYDLVA GSNCLKSSYV LSKSRALEHF PMLQKDKLVG AIVYYDGQHN DARMNLAIAL TAARYGAATA NYMEVVSLLK KTDPQTGKVR VSGARCKDVL TGQEFDVRAK CVINATGPFT DSVRKMDDKD AAAICQPSAG VHIVMPGYYS P. It is sometimes possible for the material contained within the vial of "GPD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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