Western Blot (WB)
(Western Blot (WB) analysis of GPD2 polyclonal antibody)
Rabbit GPD2 Polyclonal Antibody | anti-GPD2 antibody
GPD2 Polyclonal Antibody
Gene Names
GPD2; GDH2; GPDM; mGPDH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
GPD2; Polyclonal Antibody; GPD2 Polyclonal Antibody; Glycerol-3-phosphate dehydrogenase; mitochondrial; GPD-M; GPDH-M; mtGPD; anti-GPD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GPD2 polyclonal antibody detects endogenous levels of GPD2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
727
Applicable Applications for anti-GPD2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant full length Human GPD2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of GPD2 polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of GPD2 polyclonal antibody)
Related Product Information for anti-GPD2 antibody
GPD2 (glycerol-3-phosphate dehydrogenase 2, mitochondrial), also known as GDH2 or GPDM, is a 727 amino acid protein belonging to the FAD-dependent glycerol-3-phosphate dehydrogenase family. GPD2 is involved in the conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) while reducing enzyme-bound FAD. Localizing to the outer surface of the inner mitochondrial membrane, GPD2 acts in conjunction with GPD1 (a cytosolic NAD-linked GPD) to form a glycerol phosphate shuttle that ultimately results in the reoxidation of NADH formed during glycolysis. While widely expressed in adult and fetal tissue, GPD2 is found at highest levels in human pancreatic islets where it is essential for pancreatic B-cell glucose-sensory function. Decreased levels of GPD2 leads to impaired glucose-stimulated insulin release in noninsulin-dependent diabetes mellitus. Existing as two alternatively spliced isoforms, GPD2 contains two EF-hand domains and maps to human chromosome 2q24.1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 81kDa
NCBI Official Full Name
glycerol-3-phosphate dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
glycerol-3-phosphate dehydrogenase 2
NCBI Official Symbol
GPD2
NCBI Official Synonym Symbols
GDH2; GPDM; mGPDH
NCBI Protein Information
glycerol-3-phosphate dehydrogenase, mitochondrial
UniProt Protein Name
Glycerol-3-phosphate dehydrogenase, mitochondrial
Protein Family
UniProt Gene Name
GPD2
UniProt Synonym Gene Names
GPD-M; GPDH-M
UniProt Entry Name
GPDM_HUMAN
NCBI Description
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]
Research Articles on GPD2
Similar Products
Product Notes
The GPD2 gpd2 (Catalog #AAA3011624) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPD2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GPD2 gpd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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