Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein maelstrom homolog (MAEL) Standard Curve.)
Mouse Protein Maelstrom Homolog (MAEL) ELISA Kit | MAEL elisa kit
Mouse Protein Maelstrom Homolog (MAEL) ELISA Kit
Gene Names
Mael; AU019877; 4933405K18Rik
Reactivity
Mouse
Synonyms
Protein Maelstrom Homolog (MAEL); Mouse Protein Maelstrom Homolog (MAEL) ELISA Kit; MAEL; FLJ14904; RP11-102C16.1; maelstrom homolog; MAEL elisa kit
Reactivity
Mouse
Specificity
Mouse Protein maelstrom homolog (MAEL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAEL. No significant cross-reactivity or interference between Mouse MAEL and analogues was observed.
Sequence Length
434
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein maelstrom homolog (MAEL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein maelstrom homolog (MAEL) Standard Curve.)
Related Product Information for MAEL elisa kit
Description: This Mouse Protein maelstrom homolog (MAEL) ELISA Kit employs a two-site sandwich ELISA to quantitate MAEL in samples. An antibody specific for MAEL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAEL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAEL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAEL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By excision of a P element insertion in the 5-prime UTR of the Drosophila maelstrom gene, Findley et al. (2003) isolated mael(M391), a null allele of Drosophila maelstrom. They used database analysis to identify the human MAEL homolog. Confocal microscopy localized Drosophila maelstrom primarily to nuage, highly abundant particles within germline cells, and also to the nucleus and cytoplasm of germline cells. Nuclear shuttling assays showed that Drosophila maelstrom is transported between the cytoplasm and nucleus.By phenotypic characterization of Drosophila maelstrom mutants, which displayed axial patterning defects and other polarity defects, Findley et al. (2004) defined maelstrom as a spindle-class gene that affects Vasa modification.
Background: By excision of a P element insertion in the 5-prime UTR of the Drosophila maelstrom gene, Findley et al. (2003) isolated mael(M391), a null allele of Drosophila maelstrom. They used database analysis to identify the human MAEL homolog. Confocal microscopy localized Drosophila maelstrom primarily to nuage, highly abundant particles within germline cells, and also to the nucleus and cytoplasm of germline cells. Nuclear shuttling assays showed that Drosophila maelstrom is transported between the cytoplasm and nucleus.By phenotypic characterization of Drosophila maelstrom mutants, which displayed axial patterning defects and other polarity defects, Findley et al. (2004) defined maelstrom as a spindle-class gene that affects Vasa modification.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein maelstrom homolog
NCBI Official Synonym Full Names
maelstrom spermatogenic transposon silencer
NCBI Official Symbol
Mael
NCBI Official Synonym Symbols
AU019877; 4933405K18Rik
NCBI Protein Information
protein maelstrom homolog
UniProt Protein Name
Protein maelstrom homolog
Protein Family
UniProt Gene Name
Mael
UniProt Entry Name
MAEL_MOUSE
Research Articles on MAEL
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Product Notes
The Mouse MAEL mael (Catalog #AAA9717749) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717749 ELISA Kit recognizes Mouse MAEL. It is sometimes possible for the material contained within the vial of "Protein Maelstrom Homolog (MAEL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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