Rabbit GPC Polyclonal Antibody | anti-GPC antibody
Rabbit anti-Lassa virus (strain Mouse/Sierra Leone/Josiah/1976)(LASV) GPC Polyclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
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NCBI and Uniprot Product Information
Uniprot Description
Function: Stable signal peptide (SSP) is cleaved but is apparently retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational cleavage of GP1 and GP2, glycoprotein transport to the cell plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion. Ref.6 Ref.8Glycoprotein G1 mediates virus attachment to host receptor alpha-dystroglycan DAG1. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis. Ref.6 Ref.8Glycoprotein G2 is a class I viral fusion protein, that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversable conformational changes induced upon acidification in the endosome
By similarity. Ref.6 Ref.8
Subunit structure: Glycoprotein G1 is a homotrimer; disulfide-linked. Glycoprotein G2 is a homotrimer. GP2 homotrimers bind with GP1 homotrimers to form, with the stable signal peptide, the GP-complex. Interacts with host DAG1. In infected cell, this interaction reduces the expression of functional host DAG1. GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions supposely induce virion budding. Ref.4 Ref.6
Subcellular location: Stable signal peptide: Virion membrane; Multi-pass membrane protein
Potential. Host cell membrane; Multi-pass membrane protein
Potential. Glycoprotein G2: Virion membrane; Single-pass membrane protein
Potential. Host cell membrane; Single-pass membrane protein
Potential. Note: Binding to the stable signal peptide masks endogenous ER localization signals in the cytoplasmic domain of G2 to ensure that only the fully assembled, tripartite GP complex is transported for virion assembly
By similarity.
Domain: The cytoplasmic domain of GP2 binds to the SSP.
Post-translational modification: Cleavage by signal peptidase releases the stable signal peptide, which stays associated to the glycoprotein complex through interaction with glycoprotein G2.Specific enzymatic cleavages in vivo yield mature proteins. GP-C polyprotein is cleaved in the endoplasmic reticulum by the host protease MBTPS1. Only cleaved glycoprotein is incorporated into virions
By similarity. Ref.3
Sequence similarities: Belongs to the arenaviridae GPC protein family.
Research Articles on GPC
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Product Notes
The GPC gpc (Catalog #AAA7164792) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Lassa virus (strain Mouse/Sierra Leone/Josiah/1976)(LASV) GPC Polyclonal Antibody reacts with Lassa virus (strain Mouse/Sierra Leone/Josiah/1976)(LASV) and may cross-react with other species as described in the data sheet. AAA Biotech's GPC can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the GPC gpc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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