Western Blot (WB)
(GPAM Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GPAM antibody detected the GPAM protein (arrow).)
Rabbit anti-Human GPAM Polyclonal Antibody | anti-GPAM antibody
GPAM, ID (GPAM, GPAT1, KIAA1560, Glycerol-3-phosphate acyltransferase 1, mitochondrial)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Western Blot (WB)
(GPAM Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GPAM antibody detected the GPAM protein (arrow).)
Western Blot (WB)
(GPAM Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GPAM antibody detected the GPAM protein (arrow).)
Flow Cytometry (FC/FACS)
(GPAM Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GPAM Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
GPAM: glycerol-3-phosphate acyltransferase is an integral multi-pass enzyme of the mitochondrial outer membrane. It performs the first step in de novo phospholipid biosynthesis. Member of the phospholipid and glycerol acyltransferase family
Protein type: Lipid Metabolism - glycerophospholipid; EC 2.3.1.15; Membrane protein, integral; Membrane protein, multi-pass; Lipid Metabolism - glycerolipid; Mitochondrial; Transferase
Chromosomal Location of Human Ortholog: 10q25.2
Cellular Component: mitochondrial outer membrane; mitochondrial inner membrane; integral to membrane; plasma membrane
Molecular Function: glycerol-3-phosphate O-acyltransferase activity
Biological Process: regulation of cytokine secretion; acyl-CoA metabolic process; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; positive regulation of multicellular organism growth; fatty acid metabolic process; positive regulation of activated T cell proliferation; phospholipid metabolic process; fatty acid homeostasis; response to glucose stimulus; phosphatidic acid biosynthetic process; CDP-diacylglycerol biosynthetic process; defense response to virus; phospholipid homeostasis
Research Articles on GPAM
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Product Notes
The GPAM gpam (Catalog #AAA6012376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPAM, ID (GPAM, GPAT1, KIAA1560, Glycerol-3-phosphate acyltransferase 1, mitochondrial) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPAM can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the GPAM gpam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPAM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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