Rabbit anti-Human GPAA1 Polyclonal Antibody | anti-GPAA1 antibody

GPAA1, NT (GPAA1, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1 protein homolog) (MaxLight 750)

Cat. Num. AAA6303423

• Gene Names: GPAA1; GAA1; hGAA1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: GPAA1; Polyclonal Antibody; NT (GPAA1; GAA1; Glycosylphosphatidylinositol anchor attachment 1 protein; GAA1 protein homolog) (MaxLight 750); GAA1 protein homolog; anti-GPAA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GPAA1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-GPAA1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 46-73 in the N-terminal region of human GPAA1, Swiss/UniProt Accession: O43292.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251 cell line lysates (35ug/lane) usingMBS6009573. This demonstrates that MBS6009573 detected the GPAA1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS6009573 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6009573 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of U251 cells using MBS6009573 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GPAA1 antibody

Antibodies; Proteins

References

1. Jiang, W.W., et al. Mol. Cancer 6, 74 (2007). 2. Olsen, J.V., et al. Cell 127(3):635-648(2006). 3. Ho, J.C., et al. Int. J. Cancer 119(6):1330-1337(2006). 4. Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005). 5. Vainauskas, S., et al. J. Biol. Chem. 279(8):6540-6545(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4504079
• NCBI GeneID: 8733
• NCBI Accession #: NP_003792.1
• NCBI GenBank Nucleotide #: NM_003801.3
• UniProt Accession #: O43292; Q9NSS0; Q9UQ31
• Molecular Weight: 67,623 Da
• NCBI Official Full Name: glycosylphosphatidylinositol anchor attachment 1 protein
• NCBI Official Synonym Full Names: glycosylphosphatidylinositol anchor attachment 1
• NCBI Official Symbol: GPAA1
• NCBI Official Synonym Symbols: GAA1; hGAA1
• NCBI Protein Information: glycosylphosphatidylinositol anchor attachment 1 protein; GAA1 protein homolog; GPI transamidase subunit; GPI anchor attachment protein 1; GPAA1P anchor attachment protein 1 homolog; glycophosphatidylinositol anchor attachment 1; anchor attachment protein
• UniProt Protein Name: Glycosylphosphatidylinositol anchor attachment 1 protein
• Protein Family: Glycosylphosphatidylinositol anchor attachment 1 protein
• UniProt Gene Name: GPAA1
• UniProt Synonym Gene Names: GAA1; GPI anchor attachment protein 1; hGAA1
• UniProt Entry Name: GPAA1_HUMAN

NCBI Description

Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq, Jul 2008]

Uniprot Description

GPAA1: Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Endoplasmic reticulum; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: endoplasmic reticulum membrane; membrane; GPI-anchor transamidase complex

Molecular Function: tubulin binding; protein binding; GPI-anchor transamidase activity

Biological Process: cellular protein metabolic process; attachment of GPI anchor to protein; protein complex assembly; C-terminal protein lipidation; post-translational protein modification; protein retention in ER