Rabbit anti-Human GORASP1 Polyclonal Antibody | anti-GORASP1 antibody

GORASP1, ID (GORASP1, GOLPH5, GRASP65, Golgi reassembly-stacking protein 1, Golgi peripheral membrane protein p65, Golgi phosphoprotein 5, Golgi reassembly-stacking protein of 65kD) (Biotin)

Cat. Num. AAA6303295

• Gene Names: GORASP1; P65; GOLPH5; GRASP65
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GORASP1; Polyclonal Antibody; ID (GORASP1; GOLPH5; GRASP65; Golgi reassembly-stacking protein 1; Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly-stacking protein of 65kD) (Biotin); Golgi reassembly-stacking protein of 65kD; anti-GORASP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GORASP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GORASP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-119 amino acids from the Central region of human GORASP1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GORASP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the GORASP1 antibody detected the GORASP1 protein (arrow).)

Related Product Information for anti-GORASP1 antibody

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined.

Product Categories/Family for anti-GORASP1 antibody

Antibodies; Golgi Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Tang, D., et al. Traffic 11(6):827-842(2010). Xiang, Y., et al. J. Cell Biol. 188(2):237-251(2010). D'Angelo, G., et al. J. Biol. Chem. 284(50):34849-34860(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 13994253
• NCBI GeneID: 64689
• NCBI Accession #: NP_114105.1
• NCBI GenBank Nucleotide #: NM_031899.3
• UniProt Accession #: Q9BQQ3; Q3SYG7; Q8N272; Q96H42; B3KWC8
• Molecular Weight: 26,237 Da
• NCBI Official Full Name: Golgi reassembly-stacking protein 1 isoform 1
• NCBI Official Synonym Full Names: golgi reassembly stacking protein 1, 65kDa
• NCBI Official Symbol: GORASP1
• NCBI Official Synonym Symbols: P65; GOLPH5; GRASP65
• NCBI Protein Information: Golgi reassembly-stacking protein 1; Golgi phosphoprotein 5; Golgi peripheral membrane protein p65; Golgi reassembly and stacking protein 1; golgi reassembly-stacking protein of 65 kDa
• UniProt Protein Name: Golgi reassembly-stacking protein 1
• Protein Family: Golgi reassembly-stacking protein
• UniProt Gene Name: GORASP1
• UniProt Synonym Gene Names: GOLPH5; GRASP65; GOLPH5; GRASP65
• UniProt Entry Name: GORS1_HUMAN

Uniprot Description

GRASP65: Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes. Belongs to the GORASP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 3p22-p21.33

Cellular Component: Golgi membrane; Golgi apparatus

Biological Process: protein transport; negative regulation of dendrite morphogenesis; mitotic cell cycle; Golgi organization and biogenesis