Mouse anti-Human GOLGA5 Polyclonal Antibody | anti-GOLGA5 antibody

GOLGA5 (Golgin Subfamily A Member 5, Cell Proliferation-inducing Gene 31 Protein, Golgin-84, Protein Ret-II, RET-fused Gene 5 Protein, RETII, RFG5, PIG31)

Cat. Num. AAA646329

• Gene Names: GOLGA5; RFG5; GOLIM5; ret-II
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: GOLGA5; Polyclonal Antibody; GOLGA5 (Golgin Subfamily A Member 5; Cell Proliferation-inducing Gene 31 Protein; Golgin-84; Protein Ret-II; RET-fused Gene 5 Protein; RETII; RFG5; PIG31); Anti -GOLGA5 (Golgin Subfamily A Member 5; anti-GOLGA5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GOLGA5.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSWFVDLAGKAEDLLNRVDQGAATALSRKDNASNIYSKNTDYTELHQQNTDLIYQTGPKSTYISSAADNIRNQKATILAGTANVKVGSRTPVEASHPVENASVPRPSSHFVRRKKSEPDDELLFDFLNSSQKEPTGRVEIRKEKGKTPVFQSSQTSSVSSVNPSVTTIKTIEENSFGSQTHEAASNSDSSHEGQEESSKENVSSNAACPDHTPTPNDDGKSHELSNLRLENQLLRNEVQSLNQEMASLLQRSKETQEELNKARARVEKWNADHSKSDRMTRGLRAQVDDLTEAVAAKDSQLAVLKVRLQEADQLLSTRTEALEALQSEKSRIMQDQSEGNSLQNQALQTFQERLHEADATLKREQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDELQQQVKLYKLNLESSKQELIDYKQKATRILQSKEKLINSLKEGSGFEGLDSSTASSMELEELRHEKEMQREEIQKLMGQIHQLRSELQDMEAQQVNEAESAREQLQDLHDQIAGQKASKQELETELERLKQEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQLTNKTLSNSSQSELENRLHQLTETLIQKQTMLESLSTEKNSLVFQLERLEQQMNSASGSSSNGSSINMSGIDNGEGTRLRNVPVLFNDTETNLAGMYGKVRKAASSIDQFSIRLGIFLRRYPIARVFVIIYMALLHLWVMIVLLTYTPEMHHDQPYGK

• Applicable Applications for anti-GOLGA5 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human GOLGA5, aa1-731 (NP_005104.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MBS646329: Western Blot analysis of GOLGA5 expression in human liver.)

Western Blot (WB)

(Western Blot analysis of GOLGA5 expression using MBS646329 in HepG2.)

Western Blot (WB)

(Western Blot analysis of GOLGA5 expression in transfected 293T cell line by MBS646329. Lane 1: GOLGA5 transfected lysate (80.41KD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of MBS646329 (10ug/ml) on HeLa cell.)

Related Product Information for anti-GOLGA5 antibody

Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.

Product Categories/Family for anti-GOLGA5 antibody

Antibodies; Abs to Golgi Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 526118265
• NCBI GeneID: 9950
• NCBI Accession #: NP_005104.3
• NCBI GenBank Nucleotide #: NM_005113.3
• UniProt Accession #: Q8TBA6; O95287; Q03962; Q2TS49; Q9UQQ7; C9JRU1
• Molecular Weight: 83,024 Da
• NCBI Official Full Name: Golgin subfamily A member 5
• NCBI Official Synonym Full Names: golgin A5
• NCBI Official Symbol: GOLGA5
• NCBI Official Synonym Symbols: RFG5; GOLIM5; ret-II
• NCBI Protein Information: Golgin subfamily A member 5; golgin-84; RET-fused gene 5 protein; golgi integral membrane protein 5; golgi autoantigen, golgin subfamily a, 5; cell proliferation-inducing gene 31 protein
• UniProt Protein Name: Golgin subfamily A member 5
• Protein Family: Golgin subfamily
• UniProt Gene Name: GOLGA5
• UniProt Synonym Gene Names: RETII; RFG5
• UniProt Entry Name: GOGA5_HUMAN

NCBI Description

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport. Ref.10 Ref.13

Subunit structure: Homodimer. Interacts with RAB1A that has been activated by GTP-binding, and possibly also with OCRL1. Interacts with isoform CASP of CUX1. Ref.1 Ref.10 Ref.13

Subcellular location: Golgi apparatus membrane; Single-pass type IV membrane protein. Note: Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo-vesicular structures of the cis-Golgi network. Ref.1

Tissue specificity: Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa. Ref.1

Post-translational modification: Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. Ref.10

Involvement in disease: Thyroid papillary carcinoma (TPC) [MIM:188550]: A common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells.Note: The gene represented in this entry is involved in disease pathogenesis. A chromosomal aberration involving GOLGA5 is found in thyroid papillary carcinomas. Translocation t(10;14)(q11;q32) with RET. The translocation generates the RET/GOLGA5 (PTC5) oncogene which was found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl.

Sequence caution: The sequence CAA33787.1 differs from that shown. Reason: Chimeric cDNA. A chimeric cDNA originating from chromosomes 14 and 10.