Rabbit anti-Human, Mouse GOLGA5 Polyclonal Antibody | anti-GOLGA5 antibody

GOLGA5 (Golgin Subfamily A Member 5, Cell Proliferation-inducing Gene 31 Protein, Golgin-84, Protein Ret-II, RET-fused Gene 5 Protein, RETII, RFG5, PIG31) (FITC)

Cat. Num. AAA6380124

• Gene Names: GOLGA5; RFG5; GOLIM5; ret-II
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GOLGA5; Polyclonal Antibody; GOLGA5 (Golgin Subfamily A Member 5; Cell Proliferation-inducing Gene 31 Protein; Golgin-84; Protein Ret-II; RET-fused Gene 5 Protein; RETII; RFG5; PIG31) (FITC); anti-GOLGA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GOLGA5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 2891

• Applicable Applications for anti-GOLGA5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human GOLGA5, aa1-731 (NP_005104.2).
• Immunogen Sequence: MSWFVDLAGKAEDLLNRVDQGAATALSRKDNASNIYSKNTDYTELHQQNTDLIYQTGPKSTYISSAADNIRNQKATILAGTANVKVGSRTPVEASHPVENASVPRPSSHFVRRKKSEPDDELLFDFLNSSQKEPTGRVEIRKEKGKTPVFQSSQTSSVSSVNPSVTTIKTIEENSFGSQTHEAASNSDSSHEGQEESSKENVSSNAACPDHTPTPNDDGKSHELSNLRLENQLLRNEVQSLNQEMASLLQRSKETQEELNKARARVEKWNADHSKSDRMTRGLRAQVDDLTEAVAAKDSQLAVLKVRLQEADQLLSTRTEALEALQSEKSRIMQDQSEGNSLQNQALQTFQERLHEADATLKREQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDELQQQVKLYKLNLESSKQELIDYKQKATRILQSKEKLINSLKEGSGFEGLDSSTASSMELEELRHEKEMQREEIQKLMGQIHQLRSELQDMEAQQVNEAESAREQLQDLHDQIAGQKASKQELETELERLKQEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQLTNKTLSNSSQSELENRLHQLTETLIQKQTMLESLSTEKNSLVFQLERLEQQMNSASGSSSNGSSINMSGIDNGEGTRLRNVPVLFNDTETNLAGMYGKVRKAASSIDQFSIRLGIFLRRYPIARVFVIIYMALLHLWVMIVLLTYTPEMHHDQPYGK
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GOLGA5 rabbit polyclonal antibody. Western Blot analysis of GOLGA5 expression in human placenta.)

Western Blot (WB)

(GOLGA5 rabbit polyclonal antibody. Western Blot analysis of GOLGA5 expression in mouse testis.)

Western Blot (WB)

(GOLGA5 rabbit polyclonal antibody. Western Blot analysis of GOLGA5 expression in A-431.)

Western Blot (WB)

(Western Blot analysis of GOLGA5 expression in transfected 293T cell line by GOLGA5 polyclonal antibody. Lane 1: GOLGA5 transfected lysate (83.0kD). Lane 2: Non-transfected lysate. )

Related Product Information for anti-GOLGA5 antibody

Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.

Product Categories/Family for anti-GOLGA5 antibody

Antibodies; Golgi Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 30260187
• NCBI GeneID: 9950
• NCBI Accession #: NM_005113.2
• NCBI GenBank Nucleotide #: NM_005113.2
• UniProt Accession #: Q8TBA6
• NCBI Official Full Name: Homo sapiens golgin A5 (GOLGA5), mRNA
• NCBI Official Synonym Full Names: golgin A5
• NCBI Official Symbol: GOLGA5
• NCBI Official Synonym Symbols: RFG5; GOLIM5; ret-II
• NCBI Protein Information: golgin subfamily A member 5
• UniProt Protein Name: Golgin subfamily A member 5
• Protein Family: Golgin subfamily
• UniProt Gene Name: GOLGA5
• UniProt Synonym Gene Names: RETII; RFG5
• UniProt Entry Name: GOGA5_HUMAN

NCBI Description

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013]

Uniprot Description

GOLGA5: a member of the golgin family of proteins, whose members localize to the Golgi. Involved in regulating the architecture of the Golgi apparatus. A coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues. Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. Two splice variant isoforms have been described.

Protein type: Membrane protein, integral; Cytoskeletal

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: Golgi membrane; Golgi apparatus; Golgi cisterna; cis-Golgi network; membrane; integral to membrane

Molecular Function: protein homodimerization activity; Rab GTPase binding

Biological Process: Golgi organization and biogenesis; Golgi vesicle transport

Disease: Thyroid Carcinoma, Papillary