Goat F(ab')2 Anti-Rat IgG (H+L chain specific) Secondary Antibody
Goat F(ab')2 Anti-Rat IgG (H+L), Mouse ads-BIOT
Reactivity
Rat
Applications
ELISA, Immunoblot, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Gel filtration of pepsin-digested antibody.
Synonyms
Goat F(ab')2 Anti-Rat IgG (H+L chain specific); Polyclonal Secondary Antibody; Goat F(ab')2 Anti-Rat IgG (H+L); Mouse ads-BIOT; Goat F(ab')2 Anti-Rat IgG(H+L); Mouse ads - Biotin; Goat F(ab')2 Anti-Rat IgG (H+L chain specific) (Absorbed against mouse immunoglobulins); Goat F(ab')2 Anti-Rat IgG (H+L chain specific) secondary antibody
Host
Goat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with mouse immunoglobulins.
Purity/Purification
Gel filtration of pepsin-digested antibody.
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for Goat F(ab')2 Anti-Rat IgG (H+L chain specific) secondary antibody
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry
Application Notes
Immunofluorescence Suggested Dilution: <= 1 ug/10^6 cells
ELISA Suggested Dilution: 1:5,000-1:20,000
ELISA Suggested Dilution: 1:5,000-1:20,000
Source
Pepsin digest of goat anti-rat IgG (H+L).
Cross Absorption
Pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins..
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) and Texas Red (TXRD) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Texas Red (R-PE-TXRD), and R-phycoerythrin- Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) and Texas Red (TXRD) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Texas Red (R-PE-TXRD), and R-phycoerythrin- Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Product Categories/Family for Goat F(ab')2 Anti-Rat IgG (H+L chain specific) secondary antibody
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Product Notes
The Goat F(ab')2 Anti-Rat IgG (H+L chain specific) (Catalog #AAA674719) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat F(ab')2 Anti-Rat IgG (H+L), Mouse ads-BIOT reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Goat F(ab')2 Anti-Rat IgG (H+L chain specific) can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry. Immunofluorescence Suggested Dilution: <= 1 ug/10^6 cells ELISA Suggested Dilution: 1:5,000-1:20,000. Researchers should empirically determine the suitability of the Goat F(ab')2 Anti-Rat IgG (H+L chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat F(ab')2 Anti-Rat IgG (H+L chain specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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