Western Blot (WB)
(Western Blot analysis of Recombinant EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Protein (Fc Tag) (with 50ng and 10ng) using Anti-Ebola virus EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Polyclonal Antibody at dilution of 1:1000.)
Mouse Ebola virus EBOV Glycoprotein/GP-RBD Polyclonal Antibody | anti-EBOV antibody
Anti-Ebola virus EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Polyclonal Antibody
Reactivity
Ebola virus
Applications
Western Blot, ELISA
Purity
Protein A Affinity
Synonyms
Ebola virus EBOV Glycoprotein/GP-RBD; Polyclonal Antibody; Anti-Ebola virus EBOV (subtype Bundibugyo; strainuganda 2007) Glycoprotein/GP-RBD Polyclonal Antibody; Glycoprotein; GP; anti-EBOV antibody
Host
Mouse
Reactivity
Ebola virus
Clonality
Polyclonal
Isotype
IgG1
Clone Number
07
Purity/Purification
Protein A Affinity
Form/Format
0.2um filtered solution in PBS
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-EBOV antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-1:5000
ELISA: 1:1000-1:2000
ELISA: 1:1000-1:2000
Immunogen
Recombinant EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Protein (Fc Tag)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Recombinant EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Protein (Fc Tag) (with 50ng and 10ng) using Anti-Ebola virus EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Recombinant EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Protein (Fc Tag) (with 50ng and 10ng) using Anti-Ebola virus EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Polyclonal Antibody at dilution of 1:1000.)
Related Product Information for anti-EBOV antibody
The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly. The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD); the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, beta-integrins, folate receptor-alpha, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry; the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Product Categories/Family for anti-EBOV antibody
Similar Products
Product Notes
The EBOV (Catalog #AAA2563750) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ebola virus EBOV (subtype Bundibugyo, strainuganda 2007) Glycoprotein/GP-RBD Polyclonal Antibody reacts with Ebola virus and may cross-react with other species as described in the data sheet. AAA Biotech's Ebola virus EBOV Glycoprotein/GP-RBD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-1:5000 ELISA: 1:1000-1:2000. Researchers should empirically determine the suitability of the EBOV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ebola virus EBOV Glycoprotein/GP-RBD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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