Goat Anti-Mouse IgG (gamma chain specific) Secondary Antibody
Goat Anti-Mouse IgG, Human ads-BGAL
Reactivity
Mouse
Purity
Affinity chromatography on pooled mouse IgG covalently linked to agarose.
Synonyms
Goat Anti-Mouse IgG (gamma chain specific); Polyclonal Secondary Antibody; Goat Anti-Mouse IgG; Human ads-BGAL; Human ads - B-Galactosidase-Labeled; Goat Anti-Mouse IgG (gamma chain specific) secondary antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with mouse IgG1, IgG2a, IgG2b, IgG2c and IgG3.
Purity/Purification
Affinity chromatography on pooled mouse IgG covalently linked to agarose.
Form/Format
The beta -galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS/NaN3 as preservative.
Application Notes
Quality tested applications include:
ELISA 1-10
FLISA
FC 5,11-13
Other referenced applications include
ELISPOT 1,3-6
IHC-FS 2,14,15
IHC-PS 16-18
CC 19-22
WB 7, 21,23-25
Multiplex 26,27
ELISA
AP conjugate 1:2,000-1: 4,000
HRP conjugate 1:4,000-1:8,000
BGAL conjugate 1:500
BIOT conjugate 1:5,000-1:20,000
FLISA
FITC,TRITC, TXRD, AF488, and AF555 conjugates 1:100-1:4000
PE, CY5, and AF647 conjugates <=1 ug/mL
Flow Cytometry
FITC, BIOT, and AF488 conjugates <= 1 ug/106 cells
PE, CY5, and AF647 conjugates <= 0.1 ug/106cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL
Other applications
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
ELISA 1-10
FLISA
FC 5,11-13
Other referenced applications include
ELISPOT 1,3-6
IHC-FS 2,14,15
IHC-PS 16-18
CC 19-22
WB 7, 21,23-25
Multiplex 26,27
ELISA
AP conjugate 1:2,000-1: 4,000
HRP conjugate 1:4,000-1:8,000
BGAL conjugate 1:500
BIOT conjugate 1:5,000-1:20,000
FLISA
FITC,TRITC, TXRD, AF488, and AF555 conjugates 1:100-1:4000
PE, CY5, and AF647 conjugates <=1 ug/mL
Flow Cytometry
FITC, BIOT, and AF488 conjugates <= 1 ug/106 cells
PE, CY5, and AF647 conjugates <= 0.1 ug/106cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL
Other applications
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Source
Pooled antisera from goats hyperimmunized with mouse IgG
Cross Absorption
Mouse IgM and IgA, pooled human sera and purified human paraproteins.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance with characteristics of a standard reference reagent.
Preparation and Storage
Store at 2-8°C or long-term at -20°C.
Product Categories/Family for Goat Anti-Mouse IgG (gamma chain specific) secondary antibody
Similar Products
Product Notes
The Goat Anti-Mouse IgG (gamma chain specific) (Catalog #AAA674385) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Mouse IgG, Human ads-BGAL reacts with Mouse and may cross-react with other species as described in the data sheet. Quality tested applications include: ELISA 1-10 FLISA FC 5,11-13 Other referenced applications include ELISPOT 1,3-6 IHC-FS 2,14,15 IHC-PS 16-18 CC 19-22 WB 7, 21,23-25 Multiplex 26,27 ELISA AP conjugate 1:2,000-1: 4,000 HRP conjugate 1:4,000-1:8,000 BGAL conjugate 1:500 BIOT conjugate 1:5,000-1:20,000 FLISA FITC,TRITC, TXRD, AF488, and AF555 conjugates 1:100-1:4000 PE, CY5, and AF647 conjugates <=1 ug/mL Flow Cytometry FITC, BIOT, and AF488 conjugates <= 1 ug/106 cells PE, CY5, and AF647 conjugates <= 0.1 ug/106cellsFor flow cytometry, the suggested use of these reagents is in a final volume of 100 uL Other applications Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. . Researchers should empirically determine the suitability of the Goat Anti-Mouse IgG (gamma chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Mouse IgG (gamma chain specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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