Goat anti Guinea Pig IgM (Fc specific) Secondary Antibody

Goat anti Guinea Pig IgM (Fc specific), conjugated with Horseradish peroxidase

Cat. Num. AAA571159

• Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunoblot, Western Blot
• Synonyms: Goat anti Guinea Pig IgM (Fc specific); Polyclonal Secondary Antibody; conjugated with Horseradish peroxidase; Goat anti Guinea Pig IgM (Fc specific) secondary antibody

• Host: Goat
• Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
• Clonality: Polyclonal
• Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgM, Fc specific
• Form/Format: HRP; Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

• Applicable Applications for Goat anti Guinea Pig IgM (Fc specific) secondary antibody: ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC-P), Dot blot, Immunoblotting.
• Source: Purified normal IgM isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Product: Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
• Product Type: Secondary Antibodies
• Preparation and Storage: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Related Product Information for Goat anti Guinea Pig IgM (Fc specific) secondary antibody

The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. For enzyme-immunocytochemical and immunohistochemical detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Product Categories/Family for Goat anti Guinea Pig IgM (Fc specific) secondary antibody

Conjugated Secondary Antibody; Veterinary