Rabbit anti-Human, Mouse GNRHR Polyclonal Antibody | anti-GNRHR antibody

GNRHR Antibody

Cat. Num. AAA9406805

• Gene Names: GNRHR; HH7; GRHR; LRHR; LHRHR; GNRHR1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: GNRHR; Polyclonal Antibody; GNRHR Antibody; gonadotropin-releasing hormone receptor; HH7; GRHR; LRHR; LHRHR; GNRHR2; anti-GNRHR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total GNRHR protein.
• Form/Format: Supplied at 4.7mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 328

• Applicable Applications for anti-GNRHR antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:10000; Western blotting: 1:2000-1:5000; Immunohistochemistry: 1:150-1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from 62-75 amino acids of Human gonadotropin-releasing hormone receptor
• Target Name: GNRHR
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGELane1: 231 cell lysateLane2: Mouse testis tissue lysateLysates: 40 ug per lanePrimary antibody: 1/2350 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 25 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 31212 (GNRHR Antibody) at dilution 1/117, on the right is treated with the synthetic peptide.)

Related Product Information for anti-GNRHR antibody

This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene.

Product Categories/Family for anti-GNRHR antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4504059
• NCBI GeneID: 2798
• NCBI Accession #: NP_000397
• NCBI GenBank Nucleotide #: NM_000406.2
• Molecular Weight: 27,727 Da
• NCBI Official Full Name: gonadotropin-releasing hormone receptor isoform 1
• NCBI Official Synonym Full Names: gonadotropin-releasing hormone receptor
• NCBI Official Symbol: GNRHR
• NCBI Official Synonym Symbols: HH7; GRHR; LRHR; LHRHR; GNRHR1
• NCBI Protein Information: gonadotropin-releasing hormone receptor
• UniProt Protein Name: Gonadotropin-releasing hormone receptor
• Protein Family: Gonadotropin-releasing hormone receptor
• UniProt Gene Name: GNRHR
• UniProt Synonym Gene Names: GRHR; GnRH receptor; GnRH-R
• UniProt Entry Name: GNRHR_HUMAN

NCBI Description

This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GNRHR: Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle- stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol- calcium second messenger system. Isoform 2 may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Defects in GNRHR are a cause of fertile eunuch syndrome (FEUNS). Fertile eunuch syndrome is a mild phenotypic form of HH going with the presence of normal testicular size and some degree of spermatogenesis. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 4q21.2

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: gonadotropin-releasing hormone receptor activity; peptide binding

Biological Process: G-protein coupled receptor protein signaling pathway; multicellular organismal development

Disease: Hypogonadotropic Hypogonadism 7 With Or Without Anosmia