Western Blot (WB)
(Western blot analysis of GNPAT Antibody (C-term) in 293 cell line lysates (35ug/lane). GNPAT (arrow) was detected using the purified Pab.)
Rabbit anti-Human GNPAT Polyclonal Antibody | anti-GNPAT antibody
GNPAT, CT (Glycerone-phosphate O-acyltransferase, Acyl-CoA:dihydroxyacetonephosphateacyltransferase, Dihydroxyacetone Phosphate Acyltransferase, DAPAT, DAP-AT, DHAPAT, DHAP-AT)
Purified by Protein A affinity chromatography
Purified by Protein A affinity chromatography
Dilution: ELISA: 1:1000
Immunohistochemistry: 1:50-1:100
Flow Cytometry:1:10-1:50
Western Blot: 1:100-1:500
Western Blot (WB)
(Western blot analysis of GNPAT Antibody (C-term) in 293 cell line lysates (35ug/lane). GNPAT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of GNPAT Antibody (C-term) in 293 cell line lysates (35ug/lane). GNPAT (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with GNPAT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with GNPAT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(GNPAT Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GNPAT Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
Catalytic activity: Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.
Pathway: Membrane lipid metabolism; glycerophospholipid metabolism.
Subunit structure: May be part of a heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT
By similarity.
Subcellular location: Peroxisome membrane; Peripheral membrane protein; Matrix side
By similarity. Note: Exclusively localized to the lumenal side of the peroxisomal membrane
By similarity.
Domain: The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate
By similarity.
Sequence similarities: Belongs to the GPAT/DAPAT family.
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Product Notes
The GNPAT gnpat (Catalog #AAA626080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GNPAT, CT (Glycerone-phosphate O-acyltransferase, Acyl-CoA:dihydroxyacetonephosphateacyltransferase, Dihydroxyacetone Phosphate Acyltransferase, DAPAT, DAP-AT, DHAPAT, DHAP-AT) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GNPAT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry and Western Blot. Dilution: ELISA: 1:1000 Immunohistochemistry: 1:50-1:100 Flow Cytometry:1:10-1:50 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the GNPAT gnpat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNPAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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