Rabbit GNAS Polyclonal Antibody | anti-GNAS antibody

GNAS Polyclonal Antibody

Cat. Num. AAA2524331

• Gene Names: Gnas; Gnas1; Gnpas; Nesp55
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: GNAS; Polyclonal Antibody; GNAS Polyclonal Antibody; AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45; anti-GNAS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1144

• Applicable Applications for anti-GNAS antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:200-1:1000; IHC: 1:25-1:100
• Immunogen: Recombinant protein of human GNAS
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Mouse brain tissue using GNAS Polyclonal Antibody at dilution of 1:500)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer using GNAS Polyclonal Antibody at dilution of 1:30)

Related Product Information for anti-GNAS antibody

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.

Product Categories/Family for anti-GNAS antibody

Cancer; Epigenetics

NCBI and Uniprot Product Information

• NCBI GI #: 253970435
• NCBI GeneID: 24896
• NCBI Accession #: NP_068617.6
• NCBI GenBank Nucleotide #: NM_021845.5
• Molecular Weight: 45,664 Da
• NCBI Official Full Name: GNAS isoform XLas
• NCBI Official Synonym Full Names: GNAS complex locus
• NCBI Official Symbol: Gnas
• NCBI Official Synonym Symbols: Gnas1; Gnpas; Nesp55
• NCBI Protein Information: ALEX; GNAS; NESP55; G-alpha-8; GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus; adenylate cyclase-stimulating G alpha protein; gnas {ECO:0000312|RGD:2716}; guanine nucleotide binding protein alpha s; guanine nucleotide-binding protein G-s, alpha subunit
• UniProt Protein Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas
• Protein Family: Protein
• UniProt Gene Name: Gnas
• UniProt Synonym Gene Names: XLalphas
• UniProt Entry Name: GNAS1_RAT

NCBI Description

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009]

Uniprot Description

G-alpha(s): a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein type: G protein, heterotrimeric alpha G(s); G protein, heterotrimeric; G protein; Oncoprotein; Vesicle; Membrane protein, peripheral

Cellular Component: dendrite; extracellular region; cytosol; lipid raft; ruffle; synaptic vesicle; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; heterotrimeric G-protein complex; intrinsic to membrane; trans-Golgi network membrane; nucleus; cell junction; sarcolemma; vesicle; endosome

Molecular Function: corticotropin-releasing hormone receptor 1 binding; GTPase activity; protein domain specific binding; GTP binding; metal ion binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; ionotropic glutamate receptor binding; insulin-like growth factor receptor binding; signal transducer activity; protein binding; mu-type opioid receptor binding; G-protein beta/gamma-subunit binding; beta-2 adrenergic receptor binding; D1 dopamine receptor binding; alpha-tubulin binding

Biological Process: skin development; developmental growth; metabolic process; positive regulation of osteoclast differentiation; G-protein signaling, adenylate cyclase activating pathway; embryonic hindlimb morphogenesis; post-embryonic development; calcium ion homeostasis; positive regulation of cAMP biosynthetic process; DNA methylation; tissue homeostasis; negative regulation of blood pressure; skeletal development; endochondral ossification; dopamine receptor, adenylate cyclase activating pathway; post-embryonic body morphogenesis; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; embryonic cranial skeleton morphogenesis; regulation of transcription from RNA polymerase II promoter; positive regulation of osteoblast differentiation; G-protein coupled receptor protein signaling pathway; cartilage development; energy reserve metabolic process; positive regulation of protein amino acid phosphorylation; cognition