Rabbit anti-Human, Mouse GNAO1 Polyclonal Antibody | anti-GNAO1 antibody

GNAO1 Polyclonal Antibody

Cat. Num. AAA3011016

• Gene Names: GNAO1; GNAO; NEDIM; EIEE17; HLA-DQB1; G-ALPHA-o
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GNAO1; Polyclonal Antibody; GNAO1 Polyclonal Antibody; DKFZp686O0962; G alpha o; G protein alpha 0; Galphao; GNAO 1; GNAO; GNAO_HUMAN; Go alpha; Guanine nucleotide binding protein (G protein) alpha activating activity polypeptide O; Guanine nucleotide binding protein alpha activating polypeptide; Guanine nucleotide binding protein G(o) subunit alpha; Guanine nucleotide-binding protein G(o) subunit alpha; anti-GNAO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GNAO1 polyclonal antibody detects endogenous levels of GNAO1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 354

• Applicable Applications for anti-GNAO1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human GNAO1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GNAO1 polyclonal antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of GNAO1 polyclonal antibody in paraffin-embedded Human Stomach tissue.)

Related Product Information for anti-GNAO1 antibody

Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an alpha-, beta- and gamma-subunit and is bound to GDP in the 'off' state. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; Galphas and Galphai bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 232133
• NCBI GeneID: 2775
• UniProt Accession #: P09471
• Molecular Weight: ~40kDa
• NCBI Official Full Name: Guanine nucleotide-binding protein G(o) subunit alpha
• NCBI Official Synonym Full Names: G protein subunit alpha o1
• NCBI Official Symbol: GNAO1
• NCBI Official Synonym Symbols: GNAO; NEDIM; EIEE17; HLA-DQB1; G-ALPHA-o
• NCBI Protein Information: guanine nucleotide-binding protein G(o) subunit alpha
• UniProt Protein Name: Guanine nucleotide-binding protein G(o) subunit alpha
• UniProt Gene Name: GNAO1
• UniProt Entry Name: GNAO_HUMAN

NCBI Description

The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

G-alpha(o): Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14. Interacts with RGS14. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, heterotrimeric alpha G((i/o/t/z)); G protein; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: neuron projection; plasma membrane; heterotrimeric G-protein complex

Molecular Function: corticotropin-releasing hormone receptor 1 binding; GTPase activity; signal transducer activity; GTP binding; mu-type opioid receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; GTPase activating protein binding; metabotropic serotonin receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; response to drug; negative regulation of calcium ion transport; response to hydrogen peroxide; muscle contraction; metabolic process; response to cytokine stimulus; forebrain development; dopamine receptor signaling pathway; response to morphine; locomotory behavior; neurite development; regulation of heart contraction; positive regulation of GTPase activity; aging

Disease: Epileptic Encephalopathy, Early Infantile, 17