Rabbit anti-Human GMPR Polyclonal Antibody | anti-GMPR antibody

GMPR, CT (GMPR, GMPR1, GMP reductase 1, Guanosine 5'-monophosphate oxidoreductase 1) (MaxLight 550)

Cat. Num. AAA6302849

• Gene Names: GMPR; GMPR1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: GMPR; Polyclonal Antibody; CT (GMPR; GMPR1; GMP reductase 1; Guanosine 5'-monophosphate oxidoreductase 1) (MaxLight 550); Guanosine 5'-monophosphate oxidoreductase 1; anti-GMPR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-GMPR antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: GMPR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-267 amino acids from the C-terminal region of human GMPR.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GMPR (arrow) using rabbit polyclonal GMPR Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GMPR gene.)

Immunohistochemistry (IHC)

(GMPR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GMPR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GMPR antibody

This gene encodes an enzyme that catalyzes the irreversible and NADPH-dependent reductive deamination of GMP to IMP. The protein also functions in the re-utilization of free intracellular bases and purine nucleosides.

Product Categories/Family for anti-GMPR antibody

Antibodies; Enzymes; Reductase

References

Chasman, D.I., et al. PLoS Genet. 5 (11), E1000730 (2009): Lamesch, P., et al. Genomics 89(3):307-315(2007). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Deng, Y., et al. Int. J. Biochem. Cell Biol. 34(9):1035-1050(2002). Salvatore, D., et al. J. Biol. Chem. 273(47):31092-31096(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 156104880
• NCBI GeneID: 2766
• NCBI Accession #: NP_006868.3
• NCBI GenBank Nucleotide #: NM_006877.3
• UniProt Accession #: P36959; Q96HQ6
• Molecular Weight: 37,419 Da
• NCBI Official Full Name: GMP reductase 1
• NCBI Official Synonym Full Names: guanosine monophosphate reductase
• NCBI Official Symbol: GMPR
• NCBI Official Synonym Symbols: GMPR1
• NCBI Protein Information: GMP reductase 1; guanine monophosphate reductase; guanosine 5'-monophosphate oxidoreductase 1; guanosine monophosphate reductase 1
• UniProt Protein Name: GMP reductase 1
• Protein Family: GMP reductase
• UniProt Gene Name: GMPR
• UniProt Synonym Gene Names: GMPR1; Guanosine monophosphate reductase 1
• UniProt Entry Name: GMPR1_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the irreversible and NADPH-dependent reductive deamination of GMP to IMP. The protein also functions in the re-utilization of free intracellular bases and purine nucleosides.[provided by RefSeq, Oct 2009]

Uniprot Description

GMPR: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides. Belongs to the IMPDH/GMPR family.

Protein type: Nucleotide Metabolism - purine; Oxidoreductase; EC 1.7.1.7

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: cytosol

Molecular Function: GMP reductase activity; metal ion binding

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleotide metabolic process; response to cold; purine base metabolic process; purine salvage