Rabbit anti-Human GMEB1 Polyclonal Antibody | anti-GMEB1 antibody

Anti-GMEB1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103943

• Gene Names: GMEB1; PIF96; P96PIF
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: GMEB1; Polyclonal Antibody; Anti-GMEB1 Antibody; Rabbit Polyclonal; GMEB1 Antibody; Rabbit PAb; Antigen Affinity Purified; glucocorticoid modulatory element binding protein 1; Anti-P96PIF Antibody; Anti-PIF96 Antibody; anti-GMEB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GMEB1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-GMEB1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the center region of the Human GMEB1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human GMEB1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human GMEB1 in human rectum with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human GMEB1 in human lung cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of GMEB1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human GMEB1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-GMEB1 antibody

Glucocorticoid modulatory element binding protein (GMEB)1 and 2 are closely related proteins that modify the glucocorticoid receptor binding locus and thus modulate glucocorticoid-mediated gene induction effects, including apoptosis. GMEB1 associates with caspases and prevents apoptosis of cells in the nervous system. Manipulating GMEB may be used therapeutically to enhance the resistance or the sensitivity to steroids. GMEB1 effectively attenuated caspase activation and apoptosis caused by these stresses in human neuroblastoma SK-N-MC cells, indicating that it functions as a potent inhibitor of caspase activation and apoptosis in response to oxidative stress. That GMEB1 blocks pro-apoptosis signals induced by a variety of stresses.

NCBI and Uniprot Product Information

• NCBI GI #: 13435375
• NCBI GeneID: 10691
• NCBI Accession #: NP_077808.1
• NCBI GenBank Nucleotide #: NM_024482.2
• UniProt Accession #: Q9Y692; Q9NWH1; Q9UKD0; B1AT48
• Molecular Weight: 61,327 Da
• NCBI Official Full Name: glucocorticoid modulatory element-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: glucocorticoid modulatory element binding protein 1
• NCBI Official Symbol: GMEB1
• NCBI Official Synonym Symbols: PIF96; P96PIF
• NCBI Protein Information: glucocorticoid modulatory element-binding protein 1; GMEB-1; PIF p96; DNA-binding protein p96PIF; parvovirus initiation factor p96
• UniProt Protein Name: Glucocorticoid modulatory element-binding protein 1
• Protein Family: Glucocorticoid modulatory element-binding protein
• UniProt Gene Name: GMEB1
• UniProt Synonym Gene Names: GMEB-1; PIF p96
• UniProt Entry Name: GMEB1_HUMAN

NCBI Description

This gene encodes a member of KDWK gene family which associates with GMEB2 protein. The GMEB1-GMEB2 complex is essential for parvovirus DNA replication. Studies in rat for a similar gene suggest that this gene's role is to modulate the transactivation of the glucocorticoid receptor when it is bound to glucocorticoid response elements. Two alternative spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Feb 2011]

Uniprot Description

GMEB1: Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription coactivator activity; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent