Rabbit anti-Human Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody | anti-GSK3a antibody

Polyclonal Antibody to Glycogen Synthase Kinase 3 Alpha (GSK3a)

Cat. Num. AAA2013023

• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Glycogen Synthase Kinase 3 Alpha (GSK3a); Polyclonal Antibody; Polyclonal Antibody to Glycogen Synthase Kinase 3 Alpha (GSK3a); anti-GSK3a antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against GSK3a. It has been selected for its ability to recognize GSK3a in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.; 　MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- QEVAYT DIKVIGNGSF GVVYQARLAE TRELVAIKKV LQDKRFKNRE LQIMRKLDHC NIVRLRYFFY SSGEKKDELY LNLVLEYVPE TVYRVARHFT KAKLTIPILY VKVYMYQLFR SLAYIHSQGV CHRDIKPQNL LVDPDTAVLK LCDFGSAKQL VRGEPNVSYI CSRYYRAPEL IFGATDYTSS IDVWSAGCVL AELLLGQPIF PGDSGVDQLV EIIKVLGTPT REQIREMNPN YTEFKFPQIK AHPWTKVFKS
• Sequence Length: 483

• Applicable Applications for anti-GSK3a antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant GSK3a (Gln115~Leu409) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2045244
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Breast cancer Tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Breast cancer Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 49574532
• NCBI GeneID: 2931
• NCBI Accession #: NP_063937.2
• NCBI GenBank Nucleotide #: NM_019884.2
• UniProt Accession #: P49840; O14959
• Molecular Weight: 50,981 Da
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: GSK3A
• UniProt Synonym Gene Names: GSK-3 alpha

NCBI Description

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

Uniprot Description

GSK3A: a constitutively active proline-directed protein kinase of the GSK family. A negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubule. Phosphorylates and inactivates glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Generally requires primed phosphorylation sites C-terminal to the targeted serine or threonine residues in its substrate. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factor deprivation. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized b-catenin mutants that lack GSK3 phosphorylation sites.

Protein type: CMGC group; EC 2.7.11.1; EC 2.7.11.26; GSK family; GSK subfamily; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: beta-catenin destruction complex; cell soma; cytosol; mitochondrion; postsynapse; proximal dendrite

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity; receptor binding; tau-protein kinase activity

Biological Process: cardiac left ventricle morphogenesis; cellular response to insulin stimulus; dopamine receptor signaling pathway; glycogen metabolic process; insulin receptor signaling pathway; IRE1-mediated unfolded protein response; negative regulation of canonical Wnt signaling pathway; negative regulation of glucose import; negative regulation of glycogen (starch) synthase activity; negative regulation of glycogen biosynthetic process; negative regulation of glycogen synthase activity, transferring glucose-1-phosphate; negative regulation of insulin receptor signaling pathway; negative regulation of TOR signaling; negative regulation of type B pancreatic cell development; negative regulation of UDP-glucose catabolic process; nervous system development; peptidyl-threonine phosphorylation; positive regulation of adrenergic receptor signaling pathway; positive regulation of amyloid-beta formation; positive regulation of cAMP biosynthetic process; positive regulation of gene expression; positive regulation of glycogen (starch) synthase activity; positive regulation of heart contraction; positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; positive regulation of protein targeting to mitochondrion; positive regulation of protein ubiquitination; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of apoptosis; regulation of systemic arterial blood pressure; Wnt signaling pathway