Immunohistochemistry (IHC)
(Immunohistochemical staining of normal human testis tissue sections using GAPDH antibody at 15 ug/ml.)
Rabbit anti-Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Polyclonal Antibody | anti-GAPDH antibody
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Immunohistochemistry (IHC)
(Immunohistochemical staining of normal human testis tissue sections using GAPDH antibody at 15 ug/ml.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of normal human testis tissue sections using GAPDH antibody at 15 ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Uniprot Description
Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules
By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Ref.6 Ref.23 Ref.40
Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Ref.6
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules
By similarity. Interacts with EIF1AD, USP25, PRKCI and WARS. Component of the GAIT complex. Ref.23 Ref.25 Ref.26 Ref.29 Ref.35 Ref.42 Ref.43
Subcellular location: Cytoplasm › cytosol. Nucleus
By similarity. Cytoplasm › perinuclear region. Membrane. Cytoplasm › cytoskeleton
By similarity. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal
By similarity. Postnuclear and Perinuclear regions. Ref.24
Post-translational modification: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus
By similarity.ISGylated
Probable. Ref.30Sulfhydration at Cys-152 increases catalytic activity
By similarity.
Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Research Articles on GAPDH
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Product Notes
The GAPDH gapdh (Catalog #AAA395964) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) can be used in a range of immunoassay formats including, but not limited to, Western Blot Loading Control (WB), ELISA (EIA). Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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