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Western Blot (WB) (Western Blot analysis of GLYAT in Mouse kidney tissue and Human kidney tissue lysates in order using GLYAT Rabbit pAb at dilution 1/300)

Rabbit anti-Human, Mouse GLYAT Polyclonal Antibody | anti-GLYAT antibody

GLYAT Rabbit pAb

Gene Names
GLYAT; CAT; GAT; ACGNAT
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
GLYAT; Polyclonal Antibody; GLYAT Rabbit pAb; glycine-N-acyltransferase; CAT; GAT; ACGNAT; anti-GLYAT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.9mg/ml (varies by lot)
Applicable Applications for anti-GLYAT antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:300
Immunogen
Fusion protein of human GLYAT
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of GLYAT in Mouse kidney tissue and Human kidney tissue lysates in order using GLYAT Rabbit pAb at dilution 1/300)

Western Blot (WB) (Western Blot analysis of GLYAT in Mouse kidney tissue and Human kidney tissue lysates in order using GLYAT Rabbit pAb at dilution 1/300)

Immunohistochemistry (IHC)

(Immunohistochemistry of GLYAT in paraffin-embedded Human ovarian cancer tissue using GLYAT Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm and Cell membrane)

Immunohistochemistry (IHC) (Immunohistochemistry of GLYAT in paraffin-embedded Human ovarian cancer tissue using GLYAT Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm and Cell membrane)
Related Product Information for anti-GLYAT antibody
The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,506 Da
NCBI Official Full Name
glycine N-acyltransferase isoform b
NCBI Official Synonym Full Names
glycine-N-acyltransferase
NCBI Official Symbol
GLYAT
NCBI Official Synonym Symbols
CAT; GAT; ACGNAT
NCBI Protein Information
glycine N-acyltransferase; AAc; HRP-1(CLP); acyl-CoA:glycine N-acyltransferase; aralkyl acyl-CoA N-acyltransferase; aralkyl acyl-CoA:amino acid N-acyltransferase; aralkyl-CoA N-acyltransferase; benzoyl-coenzyme A:glycine N-acyltransferase; glycine N-benzo
UniProt Protein Name
Glycine N-acyltransferase
Protein Family
UniProt Gene Name
GLYAT
UniProt Synonym Gene Names
ACGNAT; CAT; GAT; AAc
UniProt Entry Name
GLYAT_HUMAN

NCBI Description

The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GLYAT: Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid. Belongs to the glycine N-acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.13; Mitochondrial; EC 2.3.1.71; Transferase

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: glycine N-benzoyltransferase activity; glycine N-acyltransferase activity; transferase activity, transferring acyl groups

Biological Process: glycine metabolic process; acyl-CoA metabolic process; response to toxin; xenobiotic metabolic process; monocarboxylic acid metabolic process

Research Articles on GLYAT

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Product Notes

The GLYAT glyat (Catalog #AAA4760338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLYAT Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLYAT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:300. Researchers should empirically determine the suitability of the GLYAT glyat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLYAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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