Rabbit RGS4 Polyclonal Antibody | anti-RGS4 antibody

RGS4 Antibody (Center)

Cat. Num. AAA9214371

• Gene Names: RGS4; RGP4; SCZD9
• Reactivity: Human, mouse (Predicted Reactivity: Bovine, Monkey, Rabbit, Rat)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RGS4; Polyclonal Antibody; RGS4 Antibody (Center); Regulator of G-protein signaling 4; RGP4; anti-RGS4 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine, Monkey, Rabbit, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RGS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-174 amino acids from the Central region of human RGS4.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 147-174
• Sequence Length: 187

• Applicable Applications for anti-RGS4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of RGS4 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). RGS4 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of RGS4 Antibody (Center) in mouse brain tissue lysates (35ug/lane). RGS4 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of RGS4 (arrow) using rabbit polyclonal RGS4 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RGS4 gene.)

Related Product Information for anti-RGS4 antibody

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm.

Product Categories/Family for anti-RGS4 antibody

Cardiovascular; Signal Transduction

References

Heximer,S.P., et.al., Proc. Natl. Acad. Sci. U.S.A. 94 (26), 14389-14393 (1997)

NCBI and Uniprot Product Information

• NCBI GI #: 156416012
• NCBI GeneID: 5999
• NCBI Accession #: NP_001095915.1; NP_001106851.1; NP_001106852.1; NP_005604.1
• NCBI GenBank Nucleotide #: NM_001102445.2
• UniProt Accession #: P49798; A7XA56; A7XA58; A7XA59; A7YVV7; B1APZ3
• Molecular Weight: 23256
• NCBI Official Full Name: regulator of G-protein signaling 4 isoform 1
• NCBI Official Synonym Full Names: regulator of G-protein signaling 4
• NCBI Official Symbol: RGS4
• NCBI Official Synonym Symbols: RGP4; SCZD9
• NCBI Protein Information: regulator of G-protein signaling 4
• UniProt Protein Name: Regulator of G-protein signaling 4
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS4
• UniProt Synonym Gene Names: RGP4; RGS4
• UniProt Entry Name: RGS4_HUMAN

NCBI Description

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS4: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. Expressed in brain and heart. Expressed in brain at protein level. Expressed in prefontal and visual cortex. Isoform 4 and isoform 5 are expressed ubiquitously. Isoform 1, isoform 2 and isoform 3 are not expressed in the cerebellum. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: protein complex; cytoplasm; plasma membrane; cytosol

Molecular Function: calmodulin binding; G-protein alpha-subunit binding; GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity; inactivation of MAPK activity

Disease: Schizophrenia 9