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Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human Raji Cells; Lane3: Mouse Liver Tissue; Lane4: Mouse Heart Tissue.)
Rabbit anti-Mouse Glutathione S Transferase Kappa 1 (GSTk1) Polyclonal Antibody | anti-GSTk1 antibody
Polyclonal Antibody to Glutathione S Transferase Kappa 1 (GSTk1)
Gene Names
Gstk1; DsbA-L; AW260476; 0610025I19Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Glutathione S Transferase Kappa 1 (GSTk1); Polyclonal Antibody; Polyclonal Antibody to Glutathione S Transferase Kappa 1 (GSTk1); anti-GSTk1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GSTk1. It has been selected for its ability to recognize GSTk1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.73mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SW LGFEVLCRYQ HLWNIKLQLR PTLIAGIMKD SGNQPPAMVP RKGQYIFKEI PLLKQFFQVP LNIPKDFFGE TVKKGSINAM RFLTTVSMEQ PEMLEKVSRE IWMRVWSRDE DITEYQSILA AAVKAGMSTA QAQHFLEKIS TQQVKNKLIE NTDAACKYGA FGLPTTVAHV DGKTYMLFGS DRLELLAYLL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SW LGFEVLCRYQ HLWNIKLQLR PTLIAGIMKD SGNQPPAMVP RKGQYIFKEI PLLKQFFQVP LNIPKDFFGE TVKKGSINAM RFLTTVSMEQ PEMLEKVSRE IWMRVWSRDE DITEYQSILA AAVKAGMSTA QAQHFLEKIS TQQVKNKLIE NTDAACKYGA FGLPTTVAHV DGKTYMLFGS DRLELLAYLL
Sequence Length
226
Applicable Applications for anti-GSTk1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GSTk1 (Ser19~Ala222) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051469
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human Raji Cells; Lane3: Mouse Liver Tissue; Lane4: Mouse Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human Raji Cells; Lane3: Mouse Liver Tissue; Lane4: Mouse Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GSTk1, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GSTk1, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Brain Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,704 Da
NCBI Official Full Name
glutathione S-transferase kappa 1
NCBI Official Synonym Full Names
glutathione S-transferase kappa 1
NCBI Official Symbol
Gstk1
NCBI Official Synonym Symbols
DsbA-L; AW260476; 0610025I19Rik
NCBI Protein Information
glutathione S-transferase kappa 1
UniProt Protein Name
Glutathione S-transferase kappa 1
Protein Family
UniProt Gene Name
Gstk1
UniProt Synonym Gene Names
mGSTK1
Uniprot Description
GSTK1: Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). Belongs to the GST superfamily. Kappa family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.5.1.18; Mitochondrial; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450
Chromosomal Location of Human Ortholog: 6|6 B2.1
Cellular Component: intracellular; membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; peroxisome
Molecular Function: glutathione peroxidase activity; glutathione transferase activity; protein disulfide oxidoreductase activity; receptor binding; transferase activity
Biological Process: glutathione metabolic process
Research Articles on GSTk1
Similar Products
Product Notes
The GSTk1 gstk1 (Catalog #AAA2014311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutathione S Transferase Kappa 1 (GSTk1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione S Transferase Kappa 1 (GSTk1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GSTk1 gstk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SW LGFEVLCRYQ HLWNIKLQLR PTLIAGIMKD SGNQPPAMVP RKGQYIFKEI PLLKQFFQVP LNIPKDFFGE TVKKGSINAM RFLTTVSMEQ PEMLEKVSRE IWMRVWSRDE DITEYQSILA AAVKAGMSTA QAQHFLEKIS TQQVKNKLIE NTDAACKYGA FGLPTTVAHV DGKTYMLFGS DRLELLAYLL. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase Kappa 1 (GSTk1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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