Glutathione S Transferase Kappa 1 (GSTk1) Recombinant Protein | GSTk1 recombinant protein

Recombinant Glutathione S Transferase Kappa 1 (GSTk1)

Cat. Num. AAA2018763

• Gene Names: Gstk1; DsbA-L; AW260476; 0610025I19Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Glutathione S Transferase Kappa 1 (GSTk1); Recombinant Glutathione S Transferase Kappa 1 (GSTk1); GSTk1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SW LGFEVLCRYQ HLWNIKLQLR PTLIAGIMKD SGNQPPAMVP RKGQYIFKEI PLLKQFFQVP LNIPKDFFGE TVKKGSINAM RFLTTVSMEQ PEMLEKVSRE IWMRVWSRDE DITEYQSILA AAVKAGMSTA QAQHFLEKIS TQQVKNKLIE NTDAACKYGA FGLPTTVAHV DGKTYMLFGS DRLELLAYLLGEKWMGPVPP TA
• Sequence Length: 226

• Applicable Applications for GSTk1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser19~Ala222 (Accession # Q9DCM2) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Mitochondrion
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21313138
• NCBI GeneID: 76263
• NCBI Accession #: NP_083831.1
• NCBI GenBank Nucleotide #: NM_029555.2
• UniProt Accession #: Q9DCM2
• Molecular Weight: 27.0kDa
• NCBI Official Full Name: glutathione S-transferase kappa 1
• NCBI Official Synonym Full Names: glutathione S-transferase kappa 1
• NCBI Official Symbol: Gstk1
• NCBI Official Synonym Symbols: DsbA-L; AW260476; 0610025I19Rik
• NCBI Protein Information: glutathione S-transferase kappa 1; mGSTK1; GSTK1-1; GST 13-13; GST class-kappa; glutathione S-transferase subunit 13; glutathione S-transferase class kappa
• UniProt Protein Name: Glutathione S-transferase kappa 1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: Gstk1
• UniProt Synonym Gene Names: mGSTK1
• UniProt Entry Name: GSTK1_MOUSE

Uniprot Description

GSTK1: Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). Belongs to the GST superfamily. Kappa family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Other Amino Acids Metabolism - glutathione; Mitochondrial; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18; Transferase

Cellular Component: mitochondrion; membrane; intracellular membrane-bound organelle; mitochondrial matrix; mitochondrial inner membrane; peroxisome; intracellular

Molecular Function: transferase activity; glutathione transferase activity; protein disulfide oxidoreductase activity; glutathione peroxidase activity; receptor binding

Biological Process: glutathione metabolic process