Western Blot (WB)
(Figure: WB: GST/GRB2 probed with G8135-08 (1ug/ml))
Rabbit Glutathione S-Transferase Polyclonal Antibody | anti-GST antibody
Glutathione S-Transferase (GST) (MaxLight 405)
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3
Applications
Immunoblot, Western Blot, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Glutathione S-Transferase; Polyclonal Antibody; Glutathione S-Transferase (GST) (MaxLight 405); anti-GST antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes GST-fusion proteins.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-GST antibody
Immunoblot (IB), Western Blot (WB)
Application Notes
IB: 0.5-2ug/ml of this lot detected 100ng of MAP Kinase 2/Erk2, active. 0.5-2ug/ml of a previous lot detected 500ng of GST. Representative blot from a previous lot. Cell lysate containing GST fusion protein was resolved by electrophoresis, transferred to nitro-cellulose and probed with anti-GST (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemilumine-scence detection system. Arrow indicates the GST fusion protein.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
GST (Glutathione-S-transferase) derived from the pGEX expression vector and linked to KLH.
Conjugate
MaxLight405
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Figure: WB: GST/GRB2 probed with G8135-08 (1ug/ml))
Western Blot (WB)
(Figure: WB: GST/GRB2 probed with G8135-08 (1ug/ml))
Product Categories/Family for anti-GST antibody
References
1. Kivens, W.J., et al., Mol. Cell. Biol. 18: 5291-5307, 1998. 2. Orozco, A., et al., J. Biol. Chem. 272: 9840-9846, 1997.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23kDa
NCBI Official Full Name
glutathione S-transferase P
NCBI Official Synonym Full Names
glutathione S-transferase pi 1
NCBI Official Symbol
GSTP1
NCBI Official Synonym Symbols
PI; DFN7; GST3; GSTP; FAEES3
NCBI Protein Information
glutathione S-transferase P; GSTP1-1; GST class-pi; deafness, X-linked 7; fatty acid ethyl ester synthase III
UniProt Protein Name
Glutathione S-transferase P
Protein Family
UniProt Gene Name
GSTP1
UniProt Synonym Gene Names
FAEES3; GST3
UniProt Entry Name
GSTP1_HUMAN
Similar Products
Product Notes
The GST gstp1 (Catalog #AAA6478313) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glutathione S-Transferase can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), Western Blot (WB). IB: 0.5-2ug/ml of this lot detected 100ng of MAP Kinase 2/Erk2, active. 0.5-2ug/ml of a previous lot detected 500ng of GST. Representative blot from a previous lot. Cell lysate containing GST fusion protein was resolved by electrophoresis, transferred to nitro-cellulose and probed with anti-GST (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemilumine-scence detection system. Arrow indicates the GST fusion protein. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GST gstp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione S-Transferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
