Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GSTa3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Glutathione S Transferase Alpha 3 (GSTa3) Polyclonal Antibody | anti-GSTa3 antibody
Polyclonal Antibody to Glutathione S Transferase Alpha 3 (GSTa3)
Gene Names
Gsta3; Gst2-3
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Glutathione S Transferase Alpha 3 (GSTa3); Polyclonal Antibody; Polyclonal Antibody to Glutathione S Transferase Alpha 3 (GSTa3); anti-GSTa3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GSTa3. It has been selected for its ability to recognize GSTa3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGS-MAGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL MFQQVPMVEI DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA DLEIMILYYP HMPPEEKEAS LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG NRLSRADIAL VELLYHVEEL DPGVVDNFPL LKALRSRVSN LPTVKKFLQP GSQRKPFDDA KCVESAKKIF S
MGHHHHHHSGS-MAGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL MFQQVPMVEI DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA DLEIMILYYP HMPPEEKEAS LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG NRLSRADIAL VELLYHVEEL DPGVVDNFPL LKALRSRVSN LPTVKKFLQP GSQRKPFDDA KCVESAKKIF S
Sequence Length
222
Applicable Applications for anti-GSTa3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GSTa3 (Met1~Ser221) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2045792
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GSTa3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GSTa3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant GSTa3, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GSTa3, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,361 Da
NCBI Official Full Name
glutathione S-transferase A3 isoform a
NCBI Official Synonym Full Names
glutathione S-transferase, alpha 3
NCBI Official Symbol
Gsta3
NCBI Official Synonym Symbols
Gst2-3
NCBI Protein Information
glutathione S-transferase A3
UniProt Protein Name
Glutathione S-transferase A3
Protein Family
UniProt Gene Name
Gsta3
UniProt Synonym Gene Names
Gstyc
Uniprot Description
GSTA3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)- pregnene-3,20-dione, precursors to testosterone and progesterone, respectively. Belongs to the GST superfamily. Alpha family.
Protein type: EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450
Chromosomal Location of Human Ortholog: 1 A4|1 6.5 cM
Cellular Component: cytoplasm; cytosol
Molecular Function: glutathione transferase activity; transferase activity
Biological Process: aflatoxin catabolic process; metabolic process; ureteric bud development
Research Articles on GSTa3
Similar Products
Product Notes
The GSTa3 gsta3 (Catalog #AAA2004282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutathione S Transferase Alpha 3 (GSTa3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione S Transferase Alpha 3 (GSTa3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GSTa3 gsta3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG S-MAGKPVLH YF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL MFQQVPMVEI DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA DLEIMILYYP HMPPEEKEAS LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG NRLSRADIAL VELLYHVEEL DPGVVDNFPL LKALRSRVSN LPTVKKFLQP GSQRKPFDDA KCVESAKKIF S. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase Alpha 3 (GSTa3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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