Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Mouse Heart Tissue.)
Rabbit anti-Mouse Glutathione Peroxidase 4 (GPX4) Polyclonal Antibody | anti-GPX4 antibody
Polyclonal Antibody to Glutathione Peroxidase 4 (GPX4)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GKTDVNY TQLVDLHARY AECGLRILAF PCNQFGRQEP GSNQEIKEFA AGYNVKFDMY SKICVNGDDA HPLWKWMKVQ PKGRGMLGNA IKWNFTKFLI DKNGCVVKRY GPMEEPQVIE KDLPCYL
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Mouse Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Mouse Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GPX4, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GPX4, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. Pseudogenes of this locus have been identified on chromosomes 10 and 17. [provided by RefSeq, Aug 2017]
Uniprot Description
GPX4 iso3: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: EC 1.11.1.12; Lipid Metabolism - arachidonic acid; Mitochondrial; Nuclear envelope; Other Amino Acids Metabolism - glutathione; Oxidoreductase
Chromosomal Location of Human Ortholog: 10 C1|10 39.72 cM
Cellular Component: cytoplasm; cytosol; mitochondrial inner membrane; mitochondrion; nuclear envelope; nucleoplasm; nucleus; protein complex
Molecular Function: glutathione peroxidase activity; identical protein binding; oxidoreductase activity; peroxidase activity; phospholipid-hydroperoxide glutathione peroxidase activity; selenium binding
Biological Process: chromatin organization; glutathione metabolic process; multicellular organism development; protein polymerization; regulation of inflammatory response; response to oxidative stress; spermatogenesis
Research Articles on GPX4
Similar Products
Product Notes
The GPX4 gpx4 (Catalog #AAA2001972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutathione Peroxidase 4 (GPX4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione Peroxidase 4 (GPX4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GPX4 gpx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GKT DVNY TQLVDLHARY AECGLRILAF PCNQFGRQEP GSNQEIKEFA AGYNVKFDMY SKICVNGDDA HPLWKWMKVQ PKGRGMLGNA IKWNFTKFLI DKNGCVVKRY GPMEEPQVIE KDLPCYL. It is sometimes possible for the material contained within the vial of "Glutathione Peroxidase 4 (GPX4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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