Human Phospholipid Hydroperoxide Glutathione Peroxidase, Mitochondrial ELISA Kit | Gpx4 elisa kit

Human Phospholipid Hydroperoxide Glutathione Peroxidase, Mitochondrial ELISA Kit

Cat. Num. AAA3804304

• Gene Names: Gpx4; GPx-4; PHGPx; snGPx; GSHPx-4; mtPHGPx
• Reactivity: Human
• Synonyms: Phospholipid Hydroperoxide Glutathione Peroxidase; Mitochondrial; Human Phospholipid Hydroperoxide Glutathione Peroxidase; Mitochondrial ELISA Kit; Gpx4 elisa kit

• Reactivity: Human
• Sequence Length: 253

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 31.25 uU/mL-500 uU/mL
• Sensitivity: 1 uU/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Gpx4 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofPHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE, MITOCHONDRIALin the sample, This PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE, MITOCHONDRIALELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusPHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE, MITOCHONDRIALconcentration. The concentration ofPHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE, MITOCHONDRIALin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 90903233
• NCBI GeneID: 625249
• NCBI Accession #: NP_001032830.2
• NCBI GenBank Nucleotide #: NP_001032830.2
• UniProt Accession #: O70325
• NCBI Official Full Name: phospholipid hydroperoxide glutathione peroxidase isoform B
• NCBI Official Synonym Full Names: glutathione peroxidase 4
• NCBI Official Symbol: Gpx4
• NCBI Official Synonym Symbols: GPx-4; PHGPx; snGPx; GSHPx-4; mtPHGPx
• NCBI Protein Information: phospholipid hydroperoxide glutathione peroxidase
• UniProt Protein Name: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• Protein Family: Probable glutathione peroxidase
• UniProt Gene Name: Gpx4
• UniProt Synonym Gene Names: PHGPx; GPx-4; GSHPx-4
• UniProt Entry Name: GPX41_MOUSE

NCBI Description

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. Pseudogenes of this locus have been identified on chromosomes 10 and 17. [provided by RefSeq, Jan 2019]

Uniprot Description

GPX4 iso3: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Mitochondrial; Oxidoreductase; Other Amino Acids Metabolism - glutathione; Nuclear envelope; EC 1.11.1.12; Lipid Metabolism - arachidonic acid

Cellular Component: mitochondrion; mitochondrial inner membrane; cytoplasm; nuclear envelope; cytosol; nucleus

Molecular Function: selenium binding; peroxidase activity; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; oxidoreductase activity; glutathione peroxidase activity

Biological Process: establishment and/or maintenance of chromatin architecture; glutathione metabolic process; hydrogen peroxide catabolic process; multicellular organismal development; regulation of inflammatory response; spermatogenesis; response to oxidative stress