Rabbit anti-Human, Mouse Glutamine Synthetase Polyclonal Antibody | anti-GS antibody

Glutamine Synthetase, NT (GLNS, GLUL, GS, Glutamate Ammonia Ligase, Glutamate Decarboxylase, PIG43) (MaxLight 490)

Cat. Num. AAA6478237

• Gene Names: GLUL; GS; GLNS; PIG43; PIG59
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Glutamine Synthetase; Polyclonal Antibody; NT (GLNS; GLUL; GS; Glutamate Ammonia Ligase; Glutamate Decarboxylase; PIG43) (MaxLight 490); anti-GS antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Glutamine Synthetase. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-GS antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 70-96 in the N-terminal region of human Glutamine Synthetase. Species sequence homology: bovine, chicken, monkey, porcine
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse cerebellum tissue lysates (35ug/lane) usingMBS627619. Glutamine Synthetase (arrow) was detected usingMBS627619.)

Western Blot (WB)

(Western Blot using MBS627619 (1:2000) Lane 1: mouse brain lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit IgG at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot using MBS627619 (1:2000) Lane 1: HT-29 whole cell lysates Lane 2: mouse cerebellum lysates Lane 3: Raji whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit IgG at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human testis tissue usingMBS627619, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Product Categories/Family for anti-GS antibody

Antibodies; Enzymes; Decarboxylase

References

1. Di Tommaso, L., et.al., J. Hepatol. 50(4), 746-754 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 19923206
• NCBI GeneID: 2752
• NCBI Accession #: NP_002056.2
• NCBI GenBank Nucleotide #: NM_002065.6
• UniProt Accession #: P15104; Q499Y9; Q5T9Z1; Q7Z3W4; Q8IZ17
• Molecular Weight: 42,064 Da
• NCBI Official Full Name: glutamine synthetase
• NCBI Official Synonym Full Names: glutamate-ammonia ligase
• NCBI Official Symbol: GLUL
• NCBI Official Synonym Symbols: GS; GLNS; PIG43; PIG59
• NCBI Protein Information: glutamine synthetase; glutamine synthase; glutamate decarboxylase; proliferation-inducing protein 43; cell proliferation-inducing protein 59
• UniProt Protein Name: Glutamine synthetase
• Protein Family: Glutamine synthetase
• UniProt Gene Name: GLUL
• UniProt Synonym Gene Names: GLNS; GS
• UniProt Entry Name: GLNA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 6.3.1.2; Ligase; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: protein complex; mitochondrion; rough endoplasmic reticulum; cytoplasm; perikaryon; nerve terminal; nucleus; cytosol

Molecular Function: glutamate-ammonia ligase activity; identical protein binding; glutamate binding; dynein light chain binding; glutamate decarboxylase activity; manganese ion binding; magnesium ion binding; ATP binding

Biological Process: synaptic transmission; glutamate catabolic process; cell proliferation; glutamine biosynthetic process; response to glucose stimulus; positive regulation of insulin secretion; neurotransmitter uptake; amino acid biosynthetic process; cellular response to starvation; positive regulation of synaptic transmission, glutamatergic; protein homooligomerization; positive regulation of epithelial cell proliferation

Disease: Glutamine Deficiency, Congenital