anti-Rat Glutamate Transporter EAAT4 Polyclonal Antibody | anti-SLC1A6 antibody
Anti - Glutamate Transporter EAAT4 (GTRAP41, Excitatory Amino Acid Transporter 4)
Gene Names
SLC1A6; EAAT4
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Purified using the control peptide-Sepharose
Synonyms
Glutamate Transporter EAAT4; Polyclonal Antibody; Anti - Glutamate Transporter EAAT4 (GTRAP41; Excitatory Amino Acid Transporter 4); Glutamate Transporter EAAT4 (GTRAP41; anti-SLC1A6 antibody
Host
Host: Rabbit
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified using the control peptide-Sepharose
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
564
Applicable Applications for anti-SLC1A6 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. GTRAP41 is ~270kD protein (1).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. GTRAP41 is ~270kD protein (1).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A 15 AA synthetic peptide near the C-terminus of rat GTRAP41 was synthesized, conjugated to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SLC1A6 antibody
Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Most recently two proteins, designated GTRAP41 and GTRAP48 (for glutamate transporter EAAT4 associated protein) that specifically interact with the intracellular carboxy-terminal domain of EAAT4 have been cloned and characterized. The proteins are proposed to modulate glutamate transport activity. GTRAP41 is 2388 aa protein (~270kD). It has seventeen 16-amino acid spectrin repeats, 2 alpha-actinin domains, and a pleckstrin homology domain. GTRAP48 is 1527 aa (~170kD). GTRAP48 possesses a PDZ domain, a regulatory G protein-signaling sequence, tandem dbl homology and pleckstrin homology domains characteristic of guanine nucleotide exchange factors for the Rho family of G proteins, and 2 proline-rich sequences. brain tissue. GTRAP41 and GTRAP48 are mainly expressed in the brain, and lower levels were also detected in liver and kidney. EAAT4, GTRAP41, and GTRAP48 are all immunolocalized in cerebellar Purkinje cell soma and dendrites, with little axonal staining. GTRAP48 was found to activate Rho. Expression of GTRAP48 induced the reorganization of the actin cytoskeleton. When GTRAP41 and GTRAP48 are coexpressed, GTRAP41 colocalized with actin in structures that resemble actin-stress fibers, a typical Rho-dependent effect. Overexpression of GTRAP41 and GTRAP48 enhanced glutamate uptake. GTRAP41 and GTRAP48 may therefore enhance glutamate transport either through an increase in the catalytic rate of the transporter or through an increase in cell-surface availability or by stabilizing EAAT4 at the membrane.
Product Categories/Family for anti-SLC1A6 antibody
References
• 1. Jackson M et al (2001) Nature 410, 89-93; Ohara O et al (1998) Brain Res. Mol. Brain res. 57, 181; Stanekewich MC et al (1998) PNAS 95, 14158; Rumenapp U eat al (1999) FEBS Lett. 459, 313; Fukuhara JBC 274, 5868-5879
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
61,466 Da
NCBI Official Full Name
glutamate transporter
NCBI Official Symbol
SLC1A6
NCBI Official Synonym Symbols
EAAT4
NCBI Protein Information
excitatory amino acid transporter 4; glutamate transporter; solute carrier family 1 member 6; sodium-dependent glutamate/aspartate transporter
UniProt Protein Name
Excitatory amino acid transporter 4
Protein Family
UniProt Gene Name
SLC1A6
UniProt Synonym Gene Names
EAAT4
UniProt Entry Name
EAA4_CANFA
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Product Notes
The SLC1A6 slc1a6 (Catalog #AAA616130) is an Antibody produced from Host: Rabbit Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - Glutamate Transporter EAAT4 (GTRAP41, Excitatory Amino Acid Transporter 4) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate Transporter EAAT4 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. GTRAP41 is ~270kD protein (1). ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the SLC1A6 slc1a6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamate Transporter EAAT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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