Rabbit GluR5 Polyclonal Antibody | anti-GluR5 antibody

GluR5 (Glutamate Receptor) (HRP)

Cat. Num. AAA6478081

• Gene Names: GRIK1; EAA3; EEA3; GLR5; GLUR5; GluK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GluR5; Polyclonal Antibody; GluR5 (Glutamate Receptor) (HRP); anti-GluR5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes GluR5 isoforms, Mr 105-110kD. Species Crossreactivity: Rat. Predicted to crossreact with certain human and mouse GluR5 isoforms, based on sequence homology.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GluR5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 937-949 (CHQRRTQRKETVA) of rat GluR5-2C isoform precursor. This sequence is identical in rat isoforms GluR5-2 and GluR5-2B/GluR5-1 and human GluR5 isoform 2/EAA3A, and is not present in rat isoform GluR5-2A and human GluR5 isoform 1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: Rat brain microsomal preparation probed with G3500-30 (2ug/ml).)

Related Product Information for anti-GluR5 antibody

Glutamate receptors are the major excitatory neurotransmitter receptors in the central nervous system (CNS). They play critical roles in the mechanisms of synaptic plasticity and in the development of the nervous system. In addition, these receptors may be involved in the neuronal cell death that occurs in several neuropathological conditions.

Product Categories/Family for anti-GluR5 antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4504117
• NCBI GeneID: 2897
• NCBI Accession #: NP_000821.1
• NCBI GenBank Nucleotide #: NM_175611
• UniProt Accession #: P39086; Q13001; Q86SU9
• Molecular Weight: 103,981 Da
• NCBI Official Full Name: glutamate receptor ionotropic, kainate 1 isoform 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, kainate 1
• NCBI Official Symbol: GRIK1
• NCBI Official Synonym Symbols: EAA3; EEA3; GLR5; GLUR5; GluK1
• NCBI Protein Information: glutamate receptor ionotropic, kainate 1; gluR-5; glutamate receptor 5; excitatory amino acid receptor 3
• UniProt Protein Name: Glutamate receptor ionotropic, kainate 1
• UniProt Gene Name: GRIK1
• UniProt Synonym Gene Names: GLUR5; GluK1; EAA3; GluR-5; GluR5
• UniProt Entry Name: GRIK1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR5: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; dendrite; plasma membrane; cell junction

Molecular Function: extracellular-glutamate-gated ion channel activity; kainate selective glutamate receptor activity

Biological Process: synaptic transmission, glutamatergic; nervous system development; regulation of synaptic transmission, glutamatergic; central nervous system development; glutamate signaling pathway; positive regulation of synaptic transmission, GABAergic; negative regulation of synaptic transmission, glutamatergic; synaptic transmission; regulation of inhibitory postsynaptic membrane potential; behavioral response to pain; ionotropic glutamate receptor signaling pathway; transport; adult behavior; regulation of excitatory postsynaptic membrane potential; positive regulation of gamma-aminobutyric acid secretion