Rabbit anti-Human NCAPH Polyclonal Antibody | anti-NCAPH antibody

NCAPH, ID (NCAPH, BRRN, BRRN1, CAPH, KIAA0074, Condensin complex subunit 2, Barren homolog protein 1, Chromosome-associated protein H, Non-SMC condensin I complex subunit H, XCAP-H homolog) (AP)

Cat. Num. AAA6324067

• Gene Names: NCAPH; BRRN1; CAP-H; HCAP-H
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NCAPH; Polyclonal Antibody; ID (NCAPH; BRRN; BRRN1; CAPH; KIAA0074; Condensin complex subunit 2; Barren homolog protein 1; Chromosome-associated protein H; Non-SMC condensin I complex subunit H; XCAP-H homolog) (AP); XCAP-H homolog; anti-NCAPH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NCAPH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NCAPH antibody is generated from rabbits immunized with a KLH conjugated synthetic between 353-382 amino acids selected from the Central region of human NCAPH.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NCAPH Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NCAPH antibody detected the NCAPH protein (arrow).)

Related Product Information for anti-NCAPH antibody

This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization.

Product Categories/Family for anti-NCAPH antibody

Antibodies; KIAA; orf

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Heale, J.T., et al. Mol. Cell 21(6):837-848(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 25121987
• NCBI GeneID: 23397
• NCBI Accession #: NP_056156.2
• NCBI GenBank Nucleotide #: NM_015341.3
• UniProt Accession #: Q15003; Q8TB87
• Molecular Weight: 82,563 Da
• NCBI Official Full Name: condensin complex subunit 2
• NCBI Official Synonym Full Names: non-SMC condensin I complex, subunit H
• NCBI Official Symbol: NCAPH
• NCBI Official Synonym Symbols: BRRN1; CAP-H; HCAP-H
• NCBI Protein Information: condensin complex subunit 2; XCAP-H homolog; barren homolog 1; barren homolog protein 1; chromosome-associated protein H
• UniProt Protein Name: Condensin complex subunit 2
• Protein Family: Condensin complex
• UniProt Gene Name: NCAPH
• UniProt Synonym Gene Names: BRRN; BRRN1; CAPH; KIAA0074; hCAP-H
• UniProt Entry Name: CND2_HUMAN

NCBI Description

This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. [provided by RefSeq, Jul 2008]

Uniprot Description

NCAPH: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Belongs to the CND2 (condensin subunit 2) family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: membrane; cytoplasm; condensin complex; nucleus; cytosol

Molecular Function: protein binding

Biological Process: mitotic chromosome condensation; cell division; mitotic cell cycle