Rabbit GluR-5 Polyclonal Antibody | anti-GluR-5 antibody

GluR-5 (L12) Polyclonal Antibody

Cat. Num. AAA3000552

• Gene Names: GRIK1; EAA3; EEA3; GLR5; GLUR5; GluK1; gluR-5
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GluR-5; Polyclonal Antibody; GluR-5 (L12) Polyclonal Antibody; Glutamate receptor ionotropic; kainate 1; GluK1; Excitatory amino acid receptor 3; EAA3; Glutamate receptor 5; GluR5; GluR 5; GRIK1; GLUR5; anti-GluR-5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GluR5(L12) polyclonal antibody detects endogenous levels of GluR5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 918

• Applicable Applications for anti-GluR-5 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human GluR-5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of GluR-5 (L12) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-GluR-5 antibody

Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamategated, caution-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca2+ ions. The NMDA receptors consist of five subunits: epsilion 1, 2, 3, 4 and one zeta subunit. The zeta subunit is expressed throughout the brainstem whereas the four epsilon subunits display limited distribution.

NCBI and Uniprot Product Information

• NCBI GI #: 4504117
• NCBI GeneID: 2897
• NCBI Accession #: NP_000821.1
• NCBI GenBank Nucleotide #: NP_000821.1
• UniProt Accession #: P39086
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: glutamate receptor ionotropic, kainate 1 isoform 1
• NCBI Official Synonym Full Names: glutamate ionotropic receptor kainate type subunit 1
• NCBI Official Symbol: GRIK1
• NCBI Official Synonym Symbols: EAA3; EEA3; GLR5; GLUR5; GluK1; gluR-5
• NCBI Protein Information: glutamate receptor ionotropic, kainate 1
• UniProt Protein Name: Glutamate receptor ionotropic, kainate 1
• UniProt Gene Name: GRIK1
• UniProt Synonym Gene Names: GLUR5; GluK1; EAA3; GluR-5; GluR5
• UniProt Entry Name: GRIK1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR5: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; dendrite; plasma membrane; cell junction

Molecular Function: extracellular-glutamate-gated ion channel activity; kainate selective glutamate receptor activity

Biological Process: synaptic transmission, glutamatergic; nervous system development; regulation of synaptic transmission, glutamatergic; central nervous system development; glutamate signaling pathway; positive regulation of synaptic transmission, GABAergic; negative regulation of synaptic transmission, glutamatergic; synaptic transmission; regulation of inhibitory postsynaptic membrane potential; behavioral response to pain; ionotropic glutamate receptor signaling pathway; transport; adult behavior; regulation of excitatory postsynaptic membrane potential; positive regulation of gamma-aminobutyric acid secretion