Rabbit Glucose Transporter 5 GLUT5 Polyclonal Antibody | anti-SLC2A5 antibody

Glucose Transporter 5 GLUT5 Antibody

Cat. Num. AAA9612304

• Gene Names: SLC2A5; GLUT5; GLUT-5
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Glucose Transporter 5 GLUT5; Polyclonal Antibody; Glucose Transporter 5 GLUT5 Antibody; Facilitated glucose transporter member 5; Fructose transporter; Glucose transporte; kidney; glucose transporter like protein 5; Glucose transporter type 5; Glucose transporter type 5 small intestine; GLUT 5; GLUT-5; GLUT5; GTR5_HUMAN; SLC 2A5; SLC2A5; small intestine; solute carrier family 2 (facilitated glucose/fructose transporter); member 5; Solute carrier family 2; anti-SLC2A5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Glucose Transporter 5 GLUT5 Antibody detects endogenous levels of total Glucose Transporter 5 GLUT5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 501

• Applicable Applications for anti-SLC2A5 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Glucose Transporter 5 GLUT5, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from PC12, using Glucose Transporter 5 GLUT5 Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612304 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612304 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612304 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612304 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-SLC2A5 antibody

Function: Functions as a fructose transporter that has only low activity with other monosaccharides (PubMed:8333543, PubMed:16186102, PubMed:28083649). Can mediate the uptake of 2-deoxyglucose, but with low efficiency (PubMed:1695905). Essential for fructose uptake in the small intestine (By similarity). Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose (By similarity). Required for the development of high blood pressure in response to high dietary fructose intake (By similarity).

Subcellular Location: Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cell membrane>Sarcolemma. Note: Localized on the apical membrane of jejunum villi, but also on lateral plasma membranes of the villi. Transport to the cell membrane is dependent on RAB11A.

Tissue Specificity: Detected in skeletal muscle, and in jejunum brush border membrane and basolateral membrane (at protein level) (PubMed:7619085). Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 4507013
• NCBI GeneID: 6518
• NCBI Accession #: NP_003030.1
• NCBI GenBank Nucleotide #: NP_003030.1
• UniProt Accession #: P22732
• Molecular Weight: Observed Molecular Weight: (Observed)54kD.; Predicted Molecular Weight: (Calculated)55kDa.
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 5 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 2 member 5
• NCBI Official Symbol: SLC2A5
• NCBI Official Synonym Symbols: GLUT5; GLUT-5
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 5
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 5
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC2A5
• UniProt Synonym Gene Names: GLUT5; GLUT-5
• UniProt Entry Name: GTR5_HUMAN

NCBI Description

The protein encoded by this gene is a fructose transporter responsible for fructose uptake by the small intestine. The encoded protein also is necessary for the increase in blood pressure due to high dietary fructose consumption. [provided by RefSeq, Jun 2016]

Uniprot Description

GLUT5: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Plays an important role in fructose absorption by the intestine. Expressed primarily in the jejunal region of the small intestine. Expressed at low levels in human kidney, skeletal muscle, adipocytes, microglial cells and in the human blood-brain barrier.

Protein type: Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: fructose transmembrane transporter activity; glucose transmembrane transporter activity

Biological Process: hexose transport; fructose transport; carbohydrate metabolic process; pathogenesis; glucose transport; transmembrane transport