Rabbit anti-Human, Mouse Glucagon Polyclonal Antibody | anti-GCG antibody

Glucagon, NT (GCG, Glicentin-related Polypeptide, GRPP) (MaxLight 650)

Cat. Num. AAA6477936

• Gene Names: GCG; GLP1; GLP2; GRPP; GLP-1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Glucagon; Polyclonal Antibody; NT (GCG; Glicentin-related Polypeptide; GRPP) (MaxLight 650); Glicentin; Glucagon-like peptide 1; GLP-1; Glucagon-like peptide 1 (7-37); GLP-1 (7-37); Glucagon-like peptide 1 (7-36); GLP-1 (7-36); Glucagon-like peptide 2; GLP-2; Oxyntomodulin; OXY; OXM; anti-GCG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GCG. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-GCG antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; FLISA: 1:1000; IHC: 1:50-1:100; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion of amino acids within aa32-62 from the N-terminal region of human GCG, conjugated to KLH.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GCG antibody (N-term) in mouse bladder tissue lysates (35ug/lane). GCG (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GCG Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using GCG Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GCG antibody

Antibodies; Hormones; Steroids

References

1. Jacobo, S.M., J. Pharmacol. Exp. Ther. 330(1), 283-293 (2009). 2. Root-Bernstein, R.J. Mol. Recognit. 22(3), 177-187 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503945
• NCBI GeneID: 2641
• NCBI Accession #: NP_002045
• NCBI GenBank Nucleotide #: NM_002054.4
• UniProt Accession #: P01275; Q53TP6; A6NN65
• Molecular Weight: 12.8 kDa (112aa), confirmed by MALDI-TOF
• NCBI Official Full Name: glucagon preproprotein
• NCBI Official Synonym Full Names: glucagon
• NCBI Official Symbol: GCG
• NCBI Official Synonym Symbols: GLP1; GLP2; GRPP; GLP-1
• NCBI Protein Information: glucagon
• UniProt Protein Name: Glucagon
• Protein Family: Glucagon
• UniProt Gene Name: GCG
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
• UniProt Entry Name: GLUC_HUMAN

NCBI Description

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

Uniprot Description

GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q36-q37

Cellular Component: extracellular space; endoplasmic reticulum lumen; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; hormone activity; glucagon receptor binding; receptor binding

Biological Process: positive regulation of protein binding; positive regulation of histone H3-K4 methylation; signal transduction; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cAMP nucleotide second messenger; response to starvation; G-protein coupled receptor protein signaling pathway; cell proliferation; cellular protein metabolic process; positive regulation of protein kinase activity; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; feeding behavior; regulation of insulin secretion; negative regulation of apoptosis