Rabbit anti-Human GLTSCR2 Polyclonal Antibody | anti-NOP53 antibody

GLTSCR2 Antibody

Cat. Num. AAA9629605

• Gene Names: GLTSCR2; PICT1; PICT-1
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GLTSCR2; Polyclonal Antibody; GLTSCR2 Antibody; Glioma tumor suppressor candidate region gene 2 protein; GSCR2_HUMAN; p60; PICT1; protein interacting with carboxyl terminus 1; anti-NOP53 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: GLTSCR2 Antibody detects endogenous levels of total GLTSCR2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NOP53 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the NOP53 family.
• Post Translational Modifications: Ubiquitin-mediated proteasomal degradation is regulated by c-JUN. It is associated with relocalization to the nucleoplasm and decreased homooligomerization.Phosphorylated upon DNA damage probably by ATM and DNA-PK; may regulate NOP53 degradation.
• Subunit Structure: Homooligomer. Interacts with PTEN; regulates PTEN phosphorylation and increases its stability. Interacts with RPL11; retains RPL11 into the nucleolus. Interacts with CDKN2A/isoform tumor suppressor ARF; the interaction is direct and promotes ARF nucleoplasmic relocalization and ubiquitin-mediated proteasomal degradation. Interacts with NPM1; the interaction is direct and competitive with MYC. Interacts with NF2 (via FERM domain); the interaction is direct. Interacts with p53/TP53 (via the oligomerization region); the interaction is direct and may prevent the MDM2-mediated proteasomal degradation of p53/TP53. Interacts with DDX58; may regulate DDX58 through USP15-mediated 'Lys-63'-linked deubiquitination. Interacts with UBTF.(Microbial infection) Interacts with herpes simplex virus 1 early proteins ICP22 and ICP0.(Microbial infection) Interacts with Human herpesvirus 8 protein ORF16; may sequester ORF16 in host nucleolus and reduce its antiapoptotic activity.
• Tissue Specificity: Expressed at high levels in heart and pancreas, moderate levels in placenta, liver, skeletal muscle, and kidney, and low levels in brain and lung.
• Function: Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis. In ribosome biogenesis, may also play a role in rRNA transcription. Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions. DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation. It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation. Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway. May also inhibit cell proliferation and increase apoptosis through its interaction with NF2. May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation. Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex. May also regulate cellular aerobic respiration. In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of DDX58/RIG-1.
• Note: In the nucleolus may be more specifically localized to the fibrillar center (PubMed:27729611). Mainly nucleolar it relocalizes to the nucleoplasm under specific conditions including ribosomal stress enabling it to interact and regulate nucleoplasmic proteins like p53/TP53 (PubMed:22522597, PubMed:24923447, PubMed:27323397, PubMed:26903295). Also detected in the cytosol (PubMed:24923447, PubMed:27824081).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-NOP53 antibody

Nucleus; Nucleoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 11118656
• NCBI GeneID: 29997
• UniProt Accession #: Q9NZM5; Q9BTC6; Q9HAX6; Q9NPP1; Q9NPR4; Q9UFI2
• Molecular Weight: 54,389 Da
• NCBI Official Full Name: P60
• NCBI Official Synonym Full Names: glioma tumor suppressor candidate region gene 2
• NCBI Official Symbol: GLTSCR2
• NCBI Official Synonym Symbols: PICT1; PICT-1
• NCBI Protein Information: glioma tumor suppressor candidate region gene 2 protein; p60; protein interacting with carboxyl terminus 1
• UniProt Protein Name: Glioma tumor suppressor candidate region gene 2 protein
• Protein Family: Ribosome biogenesis protein
• UniProt Gene Name: GLTSCR2
• UniProt Entry Name: GSCR2_HUMAN

Uniprot Description

Subunit structure: Interacts with HSV-1 early proteins ICP22 and ICP0.

Subcellular location: Nucleus › nucleolus Ref.3 Ref.6.

Tissue specificity: Expressed at high levels in heart and pancreas, moderate levels in placenta, liver, skeletal muscle, and kidney, and low levels in brain and lung. Ref.1

Sequence similarities: Belongs to the GLTSCR2 family.