PRDX 3 Active Protein | PRDX3 active protein

Human Recombinant PRDX 3

Cat. Num. AAA844494

• Gene Names: PRDX3; AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: PRDX 3; Human Recombinant PRDX 3; Thioredoxin-dependent peroxide Reductase; mitochondrial; PRDX3; PRX III; Antioxidant protein 1 (AOP-1).; PRDX3 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol. ; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK PSPAASKEYF QKVNQ.
• Sequence Length: 198

• Applicable Applications for PRDX3 active protein: SDS-PAGE
• Biological Activity: Specific activity: approximately 82-83 pmole/min/ug.
• Unit Definition: Specific activity is defined as the amount of hydroperoxide that 1 ug of enzyme can reduce at 25 degree C for 1 minute.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PRDX3 active protein

Background: Peroxiredoxin-3 (Prdx3) is a mitochondrial member of the antioxidant family of thioredoxin peroxidases that uses mitochondrial thioredoxin-2 (Trx2) as a source of reducing equivalents to scavenge hydrogen peroxide (H2O2). It acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Product Categories/Family for PRDX3 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 695917253
• NCBI GeneID: 10935
• NCBI Accession #: NP_001289201.1
• NCBI GenBank Nucleotide #: NM_001302272.1
• UniProt Accession #: P30048; P35690; Q0D2H1; Q13776; Q5T5V2; Q96HK4; B2R7Z0; D3DRC9; E9PH29
• Molecular Weight: 21.5 kDa
• NCBI Official Full Name: thioredoxin-dependent peroxide reductase, mitochondrial isoform c
• NCBI Official Synonym Full Names: peroxiredoxin 3
• NCBI Official Symbol: PRDX3
• NCBI Official Synonym Symbols: AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
• NCBI Protein Information: thioredoxin-dependent peroxide reductase, mitochondrial
• UniProt Protein Name: Thioredoxin-dependent peroxide reductase, mitochondrial
• Protein Family: Thioredoxin-dependent peroxide reductase
• UniProt Gene Name: PRDX3
• UniProt Synonym Gene Names: AOP1; AOP-1; Prx-III
• UniProt Entry Name: PRDX3_HUMAN

NCBI Description

This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014]

Uniprot Description

PRDX3: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Belongs to the AhpC/TSA family.

Protein type: Oxidoreductase; EC 1.11.1.15

Chromosomal Location of Human Ortholog: 10q25-q26

Cellular Component: mitochondrion; mitochondrial matrix; early endosome; cytoplasm; IkappaB kinase complex; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; thioredoxin peroxidase activity; caspase inhibitor activity; alkyl hydroperoxide reductase activity; protein kinase binding; kinase binding

Biological Process: mitochondrion organization and biogenesis; negative regulation of kinase activity; response to lipopolysaccharide; negative regulation of caspase activity; activation of NF-kappaB transcription factor; response to reactive oxygen species; peptidyl-cysteine oxidation; regulation of mitochondrial membrane potential; response to hydrogen peroxide; hydrogen peroxide catabolic process; positive regulation of cell proliferation; myeloid cell differentiation; response to oxidative stress; maternal placenta development; negative regulation of apoptosis