Rabbit anti-Human GLIS2 Polyclonal Antibody | anti-GLIS2 antibody

GLIS2, ID (GLIS2, NKL, Zinc finger protein GLIS2, GLI-similar 2, Neuronal Krueppel-like protein) (FITC)

Cat. Num. AAA6302570

• Gene Names: GLIS2; NKL; NPHP7
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GLIS2; Polyclonal Antibody; ID (GLIS2; NKL; Zinc finger protein GLIS2; GLI-similar 2; Neuronal Krueppel-like protein) (FITC); Neuronal Krueppel-like protein; anti-GLIS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GLIS2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GLIS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-202 amino acids from the Central region of human GLIS2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GLIS2 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GLIS2 antibody detected the GLIS2 protein (arrow).)

Related Product Information for anti-GLIS2 antibody

This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear transcription factor with five C2H2-type zinc finger domains. The protein encoded by this gene is widely expressed at low levels in the neural tube and peripheral nervous system and likely promotes neuronal differentiation. It is abundantly expressed in the kidney and may have a role in the regulation of kidney morphogenesis. p120 regulates the expression level of this protein and induces the cleavage of this protein's C-terminal zinc finger domain. This protein also promotes the nuclear translocation of p120. Mutations in this gene cause nephronophthisis (NPHP), an autosomal recessive kidney disease characterized by tubular basement membrane disruption, interstitial lymphohistiocytic cell infiltration, and development of cysts at the corticomedullary border of the kidneys.

Product Categories/Family for anti-GLIS2 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Attanasio, M., et al. Nat. Genet. 39(8):1018-1024(2007). Hosking, C.R., et al. Mol. Biol. Cell 18(5):1918-1927(2007). Kim, Y.S., et al. FEBS Lett. 581(5):858-864(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Kim, Y.S., et al. Nucleic Acids Res. 31(19):5513-5525(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 110431364
• NCBI GeneID: 84662
• NCBI Accession #: NP_115964.2
• NCBI GenBank Nucleotide #: NM_032575.2
• UniProt Accession #: Q9BZE0; B3KX84
• Molecular Weight: 55,689 Da
• NCBI Official Full Name: zinc finger protein GLIS2
• NCBI Official Synonym Full Names: GLIS family zinc finger 2
• NCBI Official Symbol: GLIS2
• NCBI Official Synonym Symbols: NKL; NPHP7
• NCBI Protein Information: zinc finger protein GLIS2; GLI-similar 2; nephrocystin-7; GLI-similar protein 2; neuronal Krueppel-like protein; Kruppel-like zinc finger protein GLIS2
• UniProt Protein Name: Zinc finger protein GLIS2
• Protein Family: Zinc finger protein
• UniProt Gene Name: GLIS2
• UniProt Synonym Gene Names: NKL
• UniProt Entry Name: GLIS2_HUMAN

Uniprot Description

GLIS2: Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation. Defects in GLIS2 are the cause of nephronophthisis type 7 (NPHP7). NPHP7 is an autosomal recessive disorder resulting in end-stage renal disease during childhood or adolescence. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; metal ion binding

Biological Process: nervous system development; transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of smoothened signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell differentiation

Disease: Nephronophthisis 7