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Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Rabbit anti-Mouse Glial Fibrillary Acidic Protein Polyclonal Antibody | anti-GFAP antibody

Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)

Gene Names
Gfap; AI836096
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Glial Fibrillary Acidic Protein; Polyclonal Antibody; Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP); anti-GFAP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GFAP. It has beenselected for its ability to recognize GFAP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.37mg/ml (varies by lot)
Sequence Length
432
Applicable Applications for anti-GFAP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
GFAP (Met70~Ile374)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant GFAP (Met70~Ile374) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2036648
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Western Blot (WB)

(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Western Blot (WB) (Western Blot: Sample: Mouse Heart lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Western Blot (WB) (Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Recombinant protein)

Western Blot (WB) (Western Blot: Sample: Recombinant protein)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Brain tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Brain tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,365 Da
NCBI Official Full Name
glial fibrillary acidic protein isoform 1
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
Gfap
NCBI Official Synonym Symbols
AI836096
NCBI Protein Information
glial fibrillary acidic protein
UniProt Protein Name
Glial fibrillary acidic protein
UniProt Gene Name
Gfap
UniProt Synonym Gene Names
GFAP

Uniprot Description

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 11 E1|11 66.48 cM

Cellular Component: cell body; cell projection; cytoplasm; intermediate filament; intermediate filament cytoskeleton; intracellular; membrane; myelin sheath

Molecular Function: identical protein binding; integrin binding; kinase binding; protein binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: astrocyte development; Bergmann glial cell differentiation; extracellular matrix organization; intermediate filament organization; intermediate filament-based process; negative regulation of neuron projection development; neuron projection regeneration; positive regulation of Schwann cell proliferation; regulation of chaperone-mediated autophagy; regulation of neurotransmitter uptake; response to wounding

Research Articles on GFAP

Similar Products

Product Notes

The GFAP gfap (Catalog #AAA2026619) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glial Fibrillary Acidic Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glial Fibrillary Acidic Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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