Sheep Glial Derived Neurotrophic Factor Polyclonal Antibody | anti-GDNF antibody
Glial Derived Neurotrophic Factor (GDNF)
Serum
Serum
Dilution: Western Blot: 1:3000 using TBA (not PBS) and blocking is best performed with highest quality IgG-free BSA. Recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32kD (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCl until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq]
Uniprot Description
GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 5p13.1-p12
Cellular Component: extracellular region
Molecular Function: protein homodimerization activity; growth factor activity; receptor binding
Biological Process: axon guidance; positive regulation of dopamine secretion; nervous system development; peristalsis; adult locomotory behavior; mRNA stabilization; regulation of dopamine uptake; positive regulation of monooxygenase activity; signal transduction; enteric nervous system development; sympathetic nervous system development; ureteric bud branching; regulation of gene expression; induction of an organ; positive regulation of cell proliferation; postganglionic parasympathetic nervous system development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; positive regulation of cell differentiation; postsynaptic membrane organization; metanephros development; neural crest cell migration; neurite development; negative regulation of apoptosis
Disease: Central Hypoventilation Syndrome, Congenital; Hirschsprung Disease, Susceptibility To, 3; Pheochromocytoma
Research Articles on GDNF
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Product Notes
The GDNF gdnf (Catalog #AAA617907) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Glial Derived Neurotrophic Factor (GDNF) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glial Derived Neurotrophic Factor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:3000 using TBA (not PBS) and blocking is best performed with highest quality IgG-free BSA. Recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32kD (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCl until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6. Researchers should empirically determine the suitability of the GDNF gdnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glial Derived Neurotrophic Factor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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