Rabbit GLI2 Polyclonal Antibody | anti-GLI2 antibody

Phospho-GLI2 (Ser136) Antibody

Cat. Num. AAA9610435

• Gene Names: GLI2; CJS; HPE9; PHS2; THP1; THP2
• Reactivity: Human; Predicted: Horse, Sheep, Rabbit, Dog, Xenopus
• Applications: Western Blot, ELISA
• Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: GLI2; Polyclonal Antibody; Phospho-GLI2 (Ser136) Antibody; CJS; Gli 2; GLI family zinc finger 2; GLI Kruppel family member GLI2; GLI2_HUMAN; Glioma associated oncogene family zinc finger; HPE9; Oncogene GLI2; PHS2; Tax helper protein 1; Tax helper protein 2; Tax helper protein; Tax responsive element 2 holding protein; Tax responsive element 25 bp sequence binding protein; THP; THP1; THP2; Zinc finger protein GLI2; anti-GLI2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Horse, Sheep, Rabbit, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-GLI2(Ser136) Antibody detects endogenous levels of GLI2 only when phosphorylated at Ser136.
• Purity/Purification: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1586

• Applicable Applications for anti-GLI2 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human GLI2 around the phosphorylation site of Ser136.
• Tissue Specificity: Expressed in breast cancers (at protein level) (PubMed:26565916). Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines (at protein level) (PubMed:9557682). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines (PubMed:9557682). Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines (PubMed:9557682).
• Subcellular Location: Nucleus; Nucleus; Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of Phospho-GLI2(Ser136) in lysates of HeLa?, using Phospho-GLI2(Ser136) Antibody.)

Related Product Information for anti-GLI2 antibody

Description: Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992, PubMed:26565916). Functions as transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856).

Post Translational Modifications: Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2; this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2. Acetylation at Lys-757 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy.

Subunit Structure: Interaction with ZIC1 and ZIC2 (By similarity). Interacts with STK36 (PubMed:10806483). Interacts with SUFU; this inhibits transcriptional activation mediated by GLI2 (PubMed:24311597). Interacts (via C-terminal internal region) with FOXC1 (via N-terminus); this interaction is direct and increases GLI2 DNA-binding and transcriptional activity through a smoothened (SMO)-independent Hedgehog (Hh) signaling pathway (PubMed:26565916).

Similarity: The N-terminal domain confers transcriptional repressor activity, while the C-terminal domain mediates transcriptional activation. Belongs to the GLI C2H2-type zinc-finger protein family.

NCBI and Uniprot Product Information

• NCBI GI #: 215274258
• NCBI GeneID: 2736
• UniProt Accession #: P10070
• Molecular Weight: Observed: 133 kDa; Predicted: 168 kDa
• NCBI Official Full Name: Zinc finger protein GLI2
• NCBI Official Synonym Full Names: GLI family zinc finger 2
• NCBI Official Symbol: GLI2
• NCBI Official Synonym Symbols: CJS; HPE9; PHS2; THP1; THP2
• NCBI Protein Information: zinc finger protein GLI2
• UniProt Protein Name: Zinc finger protein GLI2
• Protein Family: Zinc finger protein
• UniProt Gene Name: GLI2
• UniProt Entry Name: GLI2_HUMAN

NCBI Description

This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]

Uniprot Description

Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992). Functions as transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856).