Rabbit anti-Human GLDN Polyclonal Antibody | anti-GLDN antibody

GLDN, ID (GLDN, COLM, Gliomedin) (MaxLight 490)

Cat. Num. AAA6302518

• Gene Names: GLDN; CLOM; COLM; CRGL2; CRG-L2; LCCS11; UNC-112
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: GLDN; Polyclonal Antibody; ID (GLDN; COLM; Gliomedin) (MaxLight 490); Gliomedin; anti-GLDN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GLDN.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-GLDN antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 271-299 in the central region of human GLDN, UniProt Accession # Q6ZMI3; NP_861454.2.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in A549 cell line lysates (35ug/lane) using 0350756. This demonstrates 0350756 detected the GLDN protein (arrow).)

Related Product Information for anti-GLDN antibody

GLDN plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier (By similarity).

Product Categories/Family for anti-GLDN antibody

Antibodies; Proteins

References

Eshed, Y., et al. Neuron 47(2):215-229(2005). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Graveel, C.R., et al. Oncogene 22(11):1730-1736(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 98986321
• NCBI GeneID: 342035
• NCBI Accession #: NP_861454.2
• NCBI GenBank Nucleotide #: NM_181789.3
• UniProt Accession #: Q6ZMI3; Q6UXZ7; Q7Z359
• Molecular Weight: 45,822 Da
• NCBI Official Full Name: gliomedin isoform 1
• NCBI Official Synonym Full Names: gliomedin
• NCBI Official Symbol: GLDN
• NCBI Official Synonym Symbols: CLOM; COLM; CRGL2; CRG-L2; LCCS11; UNC-112
• NCBI Protein Information: gliomedin
• UniProt Protein Name: Gliomedin
• Protein Family: Gliomedin
• UniProt Gene Name: GLDN
• UniProt Synonym Gene Names: COLM

NCBI Description

This gene encodes a protein that contains olfactomedin-like and collagen-like domains. The encoded protein, which exists in both transmembrane and secreted forms, promotes formation of the nodes of Ranvier in the peripheral nervous system. Mutations in this gene cause a form of lethal congenital contracture syndrome in human patients. Autoantibodies to the encoded protein have been identified in sera form patients with multifocal motor neuropathy. [provided by RefSeq, May 2017]

Uniprot Description

Ligand for NRCAM and NFASC/neurofascin that plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Mediates interaction between Schwann cell microvilli and axons via its interactions with NRCAM and NFASC. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with NRCAM, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.