Goat GJC3/connexin 29 Polyclonal Antibody | anti-GJC3 antibody
Goat anti-GJC3/connexin 29 Antibody
Gene Names
GJC3; CX29; GJE1; CX30.2; CX31.3
Reactivity
Expected from sequence similarity: Human, Dog
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GJC3/connexin 29; Polyclonal Antibody; Goat anti-GJC3/connexin 29 Antibody; GJC3; gap junction protein; gamma 3; 30.2kDa; CX30.2; CX31.3; Cx29; GJE1; connexin 29; connexin 30.2; connexin 31.3; epsilon 1; 29kDa; GJC3 antibody; 30.2kDa antibody; CX30.2 antibody; CX31.3 antibody; Cx29 antibody; GJE1 antibody; connexin 29 antibody; connexin 30.2 antibody; connexin 31.3 antibody; 29kDa antibody; Connexin 29; GJC3 / connexin 29; anti-GJC3 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
LTSESTRRHKKATDS
Sequence Length
279
Applicable Applications for anti-GJC3 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave an approx 60kDa band in Human Brain (Cerebellum) and in Human Skeletal Muscle lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.3kDa according to NP_853516.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 60kDa band in Human Brain (Cerebellum) and in Human Skeletal Muscle lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.3kDa according to NP_853516.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-LTSESTRRHKKATDS, from the internal region of the protein sequence according to NP_853516.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,299 Da
NCBI Official Full Name
gap junction gamma-3 protein
NCBI Official Synonym Full Names
gap junction protein gamma 3
NCBI Official Symbol
GJC3
NCBI Official Synonym Symbols
CX29; GJE1; CX30.2; CX31.3
NCBI Protein Information
gap junction gamma-3 protein
UniProt Protein Name
Gap junction gamma-3 protein
Protein Family
UniProt Gene Name
GJC3
UniProt Synonym Gene Names
GJE1; Cx30.2; Cx31.3
UniProt Entry Name
CXG3_HUMAN
NCBI Description
This gene encodes a gap junction protein. The encoded protein, also known as a connexin, plays a role in formation of gap junctions, which provide direct connections between neighboring cells. Mutations in this gene have been reported to be associated with nonsyndromic hearing loss.[provided by RefSeq, Feb 2010]
Uniprot Description
GJC3: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Gamma-type subfamily.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: connexon complex; integral to membrane; myelin sheath
Molecular Function: protein homodimerization activity
Biological Process: cell communication; myelination; sensory perception of sound
Research Articles on GJC3
Similar Products
Product Notes
The GJC3 gjc3 (Catalog #AAA421928) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GJC3/connexin 29 Antibody reacts with Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's GJC3/connexin 29 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave an approx 60kDa band in Human Brain (Cerebellum) and in Human Skeletal Muscle lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.3kDa according to NP_853516.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the GJC3 gjc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LTSESTRRHK KATDS. It is sometimes possible for the material contained within the vial of "GJC3/connexin 29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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