Western Blot (WB)
(Anti-Connexin 40/GJA5 antibody, MBS175364, Western blottingLane 1: Mouse Heart Tissue LysateLane 2: Mouse Heart Tissue Lysate )
Rabbit GJA5 Polyclonal Antibody | anti-GJA5 antibody
Polyclonal Anti-GJA5 Antibody
Concentration: 0.1-0.5 ug/ml
Tested Species: Ms
Predicted Species: Hu, Rat
Antigen Retrieval: -
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1 ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested
Optimal dilutions should be determined by the end users.
Western Blot (WB)
(Anti-Connexin 40/GJA5 antibody, MBS175364, Western blottingLane 1: Mouse Heart Tissue LysateLane 2: Mouse Heart Tissue Lysate )
Western Blot (WB)
(Anti-Connexin 40/GJA5 antibody, MBS175364, Western blottingLane 1: Mouse Heart Tissue LysateLane 2: Mouse Heart Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-Connexin 40/GJA5 antibody, MBS175364, IHC(P)IHC(P): Rat Cardiac Muscle Tissue )
Immunohistochemistry (IHC)
(Anti-Connexin 40/GJA5 antibody, MBS175364, IHC(P)IHC(P): Rat Cardiac Muscle Tissue )
Background: Connexin proteins oligomerize to form intercellular channels, called gap junctions, through which ions and small molecules move between adjacent cells. Connexin 40(Gap junction alpha-5 protein) is a protein that in humans is encoded by the GJA5 gene.This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
GJA5: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA5 are a cause of familial atrial standstill (FAS). Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. Defects in GJA5 are the cause of familial atrial fibrillation type 11 (ATFB11). ATFB11 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the connexin family. Alpha-type (group II) subfamily.
Protein type: Channel, misc.; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q21.1
Cellular Component: connexon complex; cell projection; integral to plasma membrane; gap junction
Molecular Function: gap junction hemi-channel activity
Biological Process: gap junction assembly; negative regulation of glomerular filtration; positive regulation of vasodilation; protein oligomerization; positive regulation of vasoconstriction; artery morphogenesis; embryonic heart tube development; negative regulation of blood pressure; angiogenesis; skeletal development; transmembrane transport; potassium ion transport; embryonic limb morphogenesis
Disease: Atrial Standstill 1; Atrial Fibrillation, Familial, 11
Research Articles on GJA5
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Product Notes
The GJA5 gja5 (Catalog #AAA175364) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Anti-GJA5 Antibody reacts with Reacts with: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GJA5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5 ug/ml Tested Species: Ms Predicted Species: Hu, Rat Antigen Retrieval: - Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1 ug/ml Tested Species: Hu, Rat Predicted Species: Ms Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested Optimal dilutions should be determined by the end users. Researchers should empirically determine the suitability of the GJA5 gja5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GJA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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