Rabbit anti-Rat Ghrelin Receptor Polyclonal Antibody | anti-GHSR antibody

Ghrelin Receptor (GHS-R)

Cat. Num. AAA610546

• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Ghrelin Receptor; Polyclonal Antibody; Ghrelin Receptor (GHS-R); Anti -Ghrelin Receptor (GHS-R); anti-GHSR antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat ghrelin receptor at ~27kD in rat brain. No crossreactivity with type 1b GHS-R. No significant sequence homology exists with other GPCRs. Species sequence homology: mouse, 100%; human, sheep, porcine, 95%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

• Applicable Applications for anti-GHSR antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot:1-10ug/ml using ECL.; ELISA: 1:10,000-1:100,000 using 50-100ng G2033-75N control peptide/well.
• Immunogen: A 20 aa peptide sequence within the cytoplasmic C-terminus of rat Grehlin Receptor (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-GHSR antibody

GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release. GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R). Ghrelin has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R. The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor. In rat stomach, a 28-aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA. The activity of both Ghrelins is the same. Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity. Rat Ghrelin is expressed in the stomach, small and large intestines, and brain regions (hypothalamic arcuate nucleus) that are involved the regulation of food intake. Both Ghrelin and GHS-R expression is detected in the heart, suggesting that Ghrelin might have some cardiovascular effects. GHS-R (rat 364aa; human 366aa, ~90% sequence homology) is a receptor for GHRP and non-peptide, low mol wt. secreatgogues. This receptor is coupled to G-a11. Two isoforms of GHS-R have been reported: isoform 1a (human 36aa) and type 1b (289aa and only 5 TM domains) appears not to bind the ghrelin. GHS-R displays the typical structure of GPCR with TM domains, extracellular N-terminus and intracellular C-terminus. It is expressed in not only in the pituitary and hypothalamus but also in the hippocampus, pancreas, and neuroendocrine tumors, including somatotropinomas and rat GH3 cells.

Product Categories/Family for anti-GHSR antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4758434
• NCBI GeneID: 2693
• NCBI Accession #: NP_004113.1
• NCBI GenBank Nucleotide #: NM_004122.2
• UniProt Accession #: Q92847; Q14D12; Q6ISR8; Q92848; Q96RJ7
• Molecular Weight: 41,329 Da
• NCBI Official Full Name: growth hormone secretagogue receptor type 1 isoform 1b
• NCBI Official Synonym Full Names: growth hormone secretagogue receptor
• NCBI Official Symbol: GHSR
• NCBI Protein Information: growth hormone secretagogue receptor type 1; GHRP; GHS-R; ghrelin receptor; GH-releasing peptide receptor
• UniProt Protein Name: Growth hormone secretagogue receptor type 1
• Protein Family: Growth hormone secretagogue receptor
• UniProt Gene Name: GHSR
• UniProt Synonym Gene Names: GHS-R; GHRP
• UniProt Entry Name: GHSR_HUMAN

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. Mutations in this gene are associated with autosomal idiopathic short stature.[provided by RefSeq, Apr 2010]

Uniprot Description

GHSR: Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine). Defects in GHSR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3q26.31

Cellular Component: neuron projection; cell surface; plasma membrane; integral to membrane; lipid raft

Molecular Function: growth hormone secretagogue receptor activity; G-protein coupled receptor activity; peptide hormone binding; growth hormone-releasing hormone receptor activity

Biological Process: response to food; positive regulation of insulin-like growth factor receptor signaling pathway; hormone-mediated signaling; response to hormone stimulus; adult feeding behavior; negative regulation of interleukin-6 biosynthetic process; positive regulation of multicellular organism growth; negative regulation of interleukin-1 beta production; decidualization; negative regulation of insulin secretion; growth hormone secretion; G-protein coupled receptor protein signaling pathway; positive regulation of fatty acid metabolic process; regulation of synaptogenesis; cellular response to insulin stimulus; positive regulation of appetite; negative regulation of inflammatory response; actin polymerization and/or depolymerization; regulation of hindgut contraction; negative regulation of tumor necrosis factor biosynthetic process

Disease: Short Stature, Idiopathic, Autosomal