Western Blot (WB)
(GGH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GGH antibody detected the GGH protein (arrow).)
Rabbit anti-Human GGH Polyclonal Antibody | anti-GGH antibody
GGH, CT (GGH, Gamma-glutamyl hydrolase, Conjugase, GH, Gamma-Glu-X carboxypeptidase) (AP)
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(GGH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GGH antibody detected the GGH protein (arrow).)
Western Blot (WB)
(GGH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GGH antibody detected the GGH protein (arrow).)
Immunohistochemistry (IHC)
(GGH Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GGH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GGH Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GGH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of GGH Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of GGH Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(GGH Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GGH Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]
Uniprot Description
GGH: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Belongs to the peptidase C26 family.
Protein type: Secreted; Cofactor and Vitamin Metabolism - folate biosynthesis; EC 3.4.19.9; Secreted, signal peptide; Protease
Chromosomal Location of Human Ortholog: 8q12.3
Cellular Component: extracellular space; lysosome; melanosome; cytosol; nucleus
Molecular Function: omega peptidase activity; exopeptidase activity
Biological Process: response to drug; response to ethanol; response to zinc ion; glutamine metabolic process; proteolysis; response to insulin stimulus
Research Articles on GGH
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Product Notes
The GGH ggh (Catalog #AAA6302083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GGH, CT (GGH, Gamma-glutamyl hydrolase, Conjugase, GH, Gamma-Glu-X carboxypeptidase) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GGH can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 ELISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GGH ggh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GGH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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