Rabbit anti-Human GFI1B Polyclonal Antibody | anti-GFI1B antibody

GFI1B Polyclonal Antibody

Cat. Num. AAA2561796

• Gene Names: GFI1B; BDPLT17; ZNF163B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Antigen Affinity Purification
• Synonyms: GFI1B; Polyclonal Antibody; GFI1B Polyclonal Antibody; GFI 1B; Gfi1b; GFI1B protein; Growth factor independent 1B protein; Growth factor independent 1B transcription repressor; Growth factor independent protein 1B; OTTHUMP00000022443; OTTHUMP00000022444; OTTHUMP000000235527; Potential regulator of CDKN1A; Potential regulator of CDKN1A translocated in CML; Translocated in CML; Zinc finger protein Gfi-1b; anti-GFI1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
• Concentration: 0.72mg/mL (varies by lot)
• Sequence Length: 330

• Applicable Applications for anti-GFI1B antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:40-1:200; ELISA: 1:5000-1:10000
• Immunogen: Synthetic peptide of human GFI1B
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using GFI1B Polyclonal Antibody at dilution of 1:40(×200))

Related Product Information for anti-GFI1B antibody

This gene encodes a zinc-finger containing transcriptional regulator that is primarily expressed in cells of hematopoietic lineage. The encoded protein complexes with numerous other transcriptional regulatory proteins including GATA-1, runt-related transcription factor 1 and histone deacetylases to control expression of genes involved in the development and maturation of erythrocytes and megakaryocytes. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet-type bleeding disorder-17. Alternate splicing results in multiple transcript variants.GFI1B (Growth Factor Independent 1B Transcriptional Repressor) is a Protein Coding gene. Diseases associated with GFI1B include Bleeding Disorder, Platelet-Type, 17 and Gray Platelet Syndrome. Among its related pathways are NF-kappaB Signaling. GO annotations related to this gene include RNA polymerase II transcription factor binding. An important paralog of this gene is GFI1.

Product Categories/Family for anti-GFI1B antibody

Cardiovascular; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 49168576
• NCBI GeneID: 8328
• UniProt Accession #: Q5VTD9
• NCBI Official Full Name: GFI1B
• NCBI Official Synonym Full Names: growth factor independent 1B transcriptional repressor
• NCBI Official Symbol: GFI1B
• NCBI Official Synonym Symbols: BDPLT17; ZNF163B
• NCBI Protein Information: zinc finger protein Gfi-1b
• UniProt Protein Name: Zinc finger protein Gfi-1b
• Protein Family: Zinc finger protein
• UniProt Gene Name: GFI1B
• UniProt Entry Name: GFI1B_HUMAN

NCBI Description

This gene encodes a zinc-finger containing transcriptional regulator that is primarily expressed in cells of hematopoietic lineage. The encoded protein complexes with numerous other transcriptional regulatory proteins including GATA-1, runt-related transcription factor 1 and histone deacetylases to control expression of genes involved in the development and maturation of erythrocytes and megakaryocytes. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet-type bleeding disorder-17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

Uniprot Description

Function: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. Ref.7 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16

Subunit structure: Component of a RCOR-GFI-KDM1A-HDAC complex. Interacts directly with RCOR1, KDM1A and HDAC2

By similarity. Forms a complex with GATA1. Interacts with histone methyltransferases EHMT2 and SUV39H1. Interacts with ARIH2 (via RING-type 2). Interacts with RUNX1T1. Ref.8 Ref.9 Ref.10 Ref.12 Ref.15

Subcellular location: Nucleus Ref.10.

Tissue specificity: Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA). Ref.1 Ref.7 Ref.14

Induction: By GATA1 which binds to GFI1B promoter in cooperation with the transcription factor NFYA. Target gene of transcription factor E2-alpha/TCF3 that promotes growth arrest and apoptosis in lymphomas. Ref.2

Domain: The zinc finger domains are essential for erythroid expansion and acts as an activation domain whereas non finger domain serves as repression domain

By similarity.The SNAG domain of GFIs is required for nuclear location and for interaction with some corepressors

By similarity.

Post-translational modification: Methylation at Lys-8 in the SNAG domain seems required for the recruitment of the corepressor complex. Ref.16

Sequence similarities: Contains 6 C2H2-type zinc fingers.